TY - JOUR
T1 - Plasma cytokine levels and human papillomavirus infection at the cervix in rural Nigerian women
AU - Mbulaiteye, S. M.
AU - Kemp, T.
AU - Gage, J. C.
AU - Ajenifuja, K. O.
AU - Kiruthu, C.
AU - Wentzensen, N. A.
AU - Adepiti, C.
AU - Wacholder, S.
AU - Burk, R. D.
AU - Schiffman, M.
AU - Pinto, L.
N1 - Funding Information:
We would like to thank Mr. David Check at the Biostatistics Branch at the National Cancer Institute (Rockville, Maryland) for help drawing the graphs. The study was supported by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Department of Health and Human Services NIH Grant # 5U01CA078527-13 & NIH contract HHSN261200900303P. The CareHPV equipment and supplies used in this study were donated by Qiagen Corporation (Gaithersburg, MD); CareHPV is not the subject of this report. The authors report no conflicts of interest.
PY - 2013/10
Y1 - 2013/10
N2 - Introduction: We conducted a study to test the hypothesis that systemic dysregulation of Th1/Th2 cytokine levels was associated with detection of carcinogenic or overall human papillomavirus (HPV) at the cervix among 964 women residing in a rural village in Nigeria. Methods: Levels in plasma were measured for 19 cytokines, including Th1-like cytokines IL-2, IL-12 (p40), TNF-a, IFN-g; Th2-like cytokines IL-4, IL-5, IL-6, IL-10, IL-13; innate/inflammation cytokines IL-1a, IL-1b, IL-8, eotaxin, MCP-1, MIP-1a, and IL-7; and cell development cytokines G-CSF, VEGF, and IL-17. Analysis was restricted to 5 cytokines, TNF-α (Th1), IL-8 (Th2), eotaxin and MCP-1 (innate/inflammation), and G-CSF (cell development), whose levels were detected in 80% or more of the samples measured as well as had a coefficient of variation of <30%. Results: Strong correlations were noted between levels of eotaxin and TNF-α (r= 0.75), IL-8 and MCP-1 (r= 0.60), eotaxin and G-CSF (r= 0.44), and G-CSF and IFN-γ (r= 0.43). Detection of carcinogenic or non-carcinogenic HPV DNA was unrelated to cytokine levels, except for levels of eotaxin and TNF-α, which were inversely correlated, albeit weakly, with detection of any carcinogenic HPV (P= 0.048 and P= 0.067, respectively). In analyses stratified by age group, levels of eotaxin were inversely correlated with detection of any HPV DNA (P= 0.026) and carcinogenic HPV (P= 0.042) in older, but not younger, women. Conclusions: Our results do not support the hypothesis of association between systemic cytokine dysregulation and detection of HPV at the cervix in Nigerian women, but subgroup analyses raise questions about inverse associations between eotaxin and TNF-α in older women.
AB - Introduction: We conducted a study to test the hypothesis that systemic dysregulation of Th1/Th2 cytokine levels was associated with detection of carcinogenic or overall human papillomavirus (HPV) at the cervix among 964 women residing in a rural village in Nigeria. Methods: Levels in plasma were measured for 19 cytokines, including Th1-like cytokines IL-2, IL-12 (p40), TNF-a, IFN-g; Th2-like cytokines IL-4, IL-5, IL-6, IL-10, IL-13; innate/inflammation cytokines IL-1a, IL-1b, IL-8, eotaxin, MCP-1, MIP-1a, and IL-7; and cell development cytokines G-CSF, VEGF, and IL-17. Analysis was restricted to 5 cytokines, TNF-α (Th1), IL-8 (Th2), eotaxin and MCP-1 (innate/inflammation), and G-CSF (cell development), whose levels were detected in 80% or more of the samples measured as well as had a coefficient of variation of <30%. Results: Strong correlations were noted between levels of eotaxin and TNF-α (r= 0.75), IL-8 and MCP-1 (r= 0.60), eotaxin and G-CSF (r= 0.44), and G-CSF and IFN-γ (r= 0.43). Detection of carcinogenic or non-carcinogenic HPV DNA was unrelated to cytokine levels, except for levels of eotaxin and TNF-α, which were inversely correlated, albeit weakly, with detection of any carcinogenic HPV (P= 0.048 and P= 0.067, respectively). In analyses stratified by age group, levels of eotaxin were inversely correlated with detection of any HPV DNA (P= 0.026) and carcinogenic HPV (P= 0.042) in older, but not younger, women. Conclusions: Our results do not support the hypothesis of association between systemic cytokine dysregulation and detection of HPV at the cervix in Nigerian women, but subgroup analyses raise questions about inverse associations between eotaxin and TNF-α in older women.
KW - Cervical cancer
KW - Cytokines
KW - Inflammation
KW - Plasmodium falciparum malaria
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U2 - 10.1016/j.cyto.2013.07.028
DO - 10.1016/j.cyto.2013.07.028
M3 - Article
C2 - 23972725
AN - SCOPUS:84883555718
SN - 1043-4666
VL - 64
SP - 146
EP - 151
JO - Cytokine
JF - Cytokine
IS - 1
ER -