TY - JOUR
T1 - Photographic and luminometric detection of luciferase reporter phages for drug susceptibility testing of clinical Mycobacterium tuberculosis isolates
AU - Hazbón, Manzour Hernando
AU - Guarín, Nora
AU - Ferro, Beatriz Eugenia
AU - Rodríguez, Ana Lucía
AU - Labrada, Luz Angela
AU - Tovar, Rafael
AU - Riska, Paul F.
AU - Jacobs, William R.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Luciferase reporter phages (LRPs) have proven to be efficient tools for drug. susceptibility testing of Mycobacterium tuberculosis. Luminometric detection of LRP activity offers higher sensitivity and quantitative results, while a Polaroid film detection method offers a "low-tech" inexpensive alternative that is called the Bronx box. In this work we evaluated, improved, and compared the performance of the luminometer and the Bronx box formats for drug susceptibility testing with LRPs by using 51 clinical isolates of M. tuberculosis, with the agar proportion method (PM) serving as reference. The sensitivity in detecting resistance to isoniazid and rifampin, antibiotics that define multidrug resistance (MDR), was 100% for both methods. The turnaround time for results was reduced from 3 weeks for PM to 54 or 94 h for luminometry or the Bronx box, respectively. These results support the utility of LRPs as a screening test for the surveillance of MDR tuberculosis.
AB - Luciferase reporter phages (LRPs) have proven to be efficient tools for drug. susceptibility testing of Mycobacterium tuberculosis. Luminometric detection of LRP activity offers higher sensitivity and quantitative results, while a Polaroid film detection method offers a "low-tech" inexpensive alternative that is called the Bronx box. In this work we evaluated, improved, and compared the performance of the luminometer and the Bronx box formats for drug susceptibility testing with LRPs by using 51 clinical isolates of M. tuberculosis, with the agar proportion method (PM) serving as reference. The sensitivity in detecting resistance to isoniazid and rifampin, antibiotics that define multidrug resistance (MDR), was 100% for both methods. The turnaround time for results was reduced from 3 weeks for PM to 54 or 94 h for luminometry or the Bronx box, respectively. These results support the utility of LRPs as a screening test for the surveillance of MDR tuberculosis.
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U2 - 10.1128/JCM.41.10.4865-4869.2003
DO - 10.1128/JCM.41.10.4865-4869.2003
M3 - Article
C2 - 14532245
AN - SCOPUS:0141889128
SN - 0095-1137
VL - 41
SP - 4865
EP - 4869
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 10
ER -