Photoacoustic imaging of the near-infrared fluorescent protein iRFP in vivo

Arie Krumholz, Grigory S. Filonov, Jun Xia, Junjie Yao, Vladislav V. Verkhusha, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Genetically encoded probes powerfully and non-invasively target specific tissues, cells, and subcellular locations. iRFP, a novel near-infrared fluorescent protein with low quantum yield whose absorption and fluorescence maxima are located at wavelengths longer than the Q-band of hemoglobin absorption, is ideal for PAT. Here, we report on an in vitro comparison of iRFP with other far-red fluorescent proteins, and its use in imaging a mouse tumor xenograft model. In an in vivo experiment, we stably transfected iRFP into MTLn3 adenocarcinoma cells and injected them into the mammary fat pad of female SCID/NCr mice, then imaged the resulting tumors two and three weeks post injection. The contrast increase from the protein expression was high enough to clearly separate the tumor region from the rest of the animal.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2012
ISBN (Print)9780819488664
StatePublished - Feb 9 2012
EventPhotons Plus Ultrasound: Imaging and Sensing 2012 - San Francisco, CA, United States
Duration: Jan 22 2012Jan 24 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherPhotons Plus Ultrasound: Imaging and Sensing 2012
Country/TerritoryUnited States
CitySan Francisco, CA


  • Deep imaging
  • Fluorescent proteins
  • Genetically encoded probe
  • IRFP
  • Photoacoustic tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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