Peptidomics: Identification and quantification of endogenous peptides in neuroendocrine tissues

Lloyd D. Fricker, Jlhyeon Lim, Hui Pan, Fa Yun Che

Research output: Contribution to journalReview articlepeer-review

180 Scopus citations


Neuropeptides perform a large variety of functions as inter-cellular signaling molecules. While most proteomic studies involve digestion of the proteins with trypsin or other proteases, peptidomics studies usually analyze the native peptide forms. Neuropeptides can be studied by using mass spectrometry for identification and quantitation. In many cases, mass spectrometry provides an understanding of the precise molecular form of the native peptide, including post-translational cleavages and other modifications. Quantitative peptidomics studies generally use differential isotopic tags to label two sets of extracted peptides, as done with proteomic studies, except that the Cys-based reagents typically used for quantitation of proteins are not suitable because most peptides lack Cys residues. Instead, a number of amine-specific labels have been created and some of these are useful for peptide quantitation by mass spectrometry. In this review, peptidomics techniques are discussed along with the major findings of many recent studies and future directions for the field.

Original languageEnglish (US)
Pages (from-to)327-344
Number of pages18
JournalMass Spectrometry Reviews
Issue number2
StatePublished - Mar 2006


  • Carboxypeptidase
  • Neuropeptide
  • Peptide processing
  • Prohormone convertase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Condensed Matter Physics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Spectroscopy


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