Paraquat toxicity and pyridine nucleotide coenzyme synthesis: A data correction

Olen R. Brown, Richard L. Seither

Research output: Contribution to journalArticlepeer-review


The decrease in pyridine nucleotide coenzymes which occurs during poisoning of Escherichia coli by hyperbaric oxygen or paraquat is not due to impairment of nicotinatemononucleotide pyrophosphorylase (carboxylating) [EC] as was previously proposed (Brown, O.R. et al. Biochem. Biophys. Res. Commun. 91:982-990; 1979). This was shown directly using extracts of E. coli, prepared after exposure to 1 mM paraquat or 4.2 atmospheres of oxygen. The enzyme also was not impaired in Neurospora crassa by 1 mM paraquat. A naturally-occurring, non-dialyzable inhibitor of the enzyme was found in E. coli extracts. The inhibitor caused the erroneous, low nicotinatemononucleotide pyrophosphorylase (carboxylating) activities previously reported in extracts of E. coli poisoned by paraquat.

Original languageEnglish (US)
Pages (from-to)113-116
Number of pages4
JournalFree Radical Biology and Medicine
Issue number2
StatePublished - 1990
Externally publishedYes


  • Free radicals
  • Nicotinatemononucleotide pyrophosphorylase (carboxylating)
  • Oxidant stress
  • Oxygen toxicity
  • Paraquat toxicity
  • Pyridine nucleotide coenzymes
  • Superoxide

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)


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