Abstract
There are 3 members of the multidrug-resitance gene family expressed in mouse. Only one of these, mdr lb, and its gene product P-glycoprotein are induced to high levels in the mouse endometrium during pregnancy. It is shown here that P-glycoprotein in the gravid uterus is significantly larger (Mr 155000) compared to P-glycoprotein encoded by mdr lb in a murine multidrug-resistant cell line (Mr 140000). However, both species co-migrate after enzymatic removal of N-linked sugars (Mr 125000). These results demonstrate that differential glycosylation of the mdr lb gene product contributes to molecular heterogeneity found in P-glycoprotein from normal and multidrug-resistant cells.
Original language | English (US) |
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Pages (from-to) | 419-421 |
Number of pages | 3 |
Journal | FEBS Letters |
Volume | 257 |
Issue number | 2 |
DOIs | |
State | Published - Nov 6 1989 |
Keywords
- Chemotherapy
- Glycosylation
- Mouse endometrium
- Multidrug resistance
- P-glycoprotein
- Pregnancy
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology