Oral microbiome alterations and sars-cov-2 saliva viral load in patients with covid-19

Emily Happy Miller; Medini K. Annavajhala; Alexander M. Chong; Heekuk Park; Yael R. Nobel; Ali Soroush; John W. Blackett; Anna Krigel; Meaghan M. Phipps; Daniel E. Freedberg; Jason Zucker; Ellen D. Sano; Anne Catrin Uhlemann; Julian A. Abrams

doi:10.1128/Spectrum.00055-21

Oral microbiome alterations and sars-cov-2 saliva viral load in patients with covid-19

Emily Happy Miller, Medini K. Annavajhala, Alexander M. Chong, Heekuk Park, Yael R. Nobel, Ali Soroush, John W. Blackett, Anna Krigel, Meaghan M. Phipps, Daniel E. Freedberg, Jason Zucker, Ellen D. Sano, Anne Catrin Uhlemann, Julian A. Abrams

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Bacterial-viral interactions in saliva have been associated with morbidity and mortality for respiratory viruses such as influenza and SARS-CoV. However, such transkingdom relationships during SARS-CoV-2 infection are currently unknown. Here, we aimed to elucidate the relationship between saliva microbiota and SARSCoV- 2 in a cohort of newly hospitalized COVID-19 patients and controls. We used 16S rRNA sequencing to compare microbiome diversity and taxonomic composition between COVID-19 patients (n = 53) and controls (n = 59) and based on saliva SARSCoV- 2 viral load as measured using reverse transcription PCR (RT-PCR). The saliva microbiome did not differ markedly between COVID-19 patients and controls. However, we identified significant differential abundance of numerous taxa based on saliva SARS-CoV-2 viral load, including multiple species within Streptococcus and Prevotella.

Original language	English (US)
Article number	e00055-21
Journal	Microbiology Spectrum
Volume	9
Issue number	2
DOIs	https://doi.org/10.1128/Spectrum.00055-21
State	Published - Oct 2021
Externally published	Yes

Keywords

COVID-19
SARS-CoV-2
Saliva microbiome
Viral load

ASJC Scopus subject areas

Physiology
Ecology
General Immunology and Microbiology
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/Spectrum.00055-21

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Miller, E. H., Annavajhala, M. K., Chong, A. M., Park, H., Nobel, Y. R., Soroush, A., Blackett, J. W., Krigel, A., Phipps, M. M., Freedberg, D. E., Zucker, J., Sano, E. D., Uhlemann, A. C., & Abrams, J. A. (2021). Oral microbiome alterations and sars-cov-2 saliva viral load in patients with covid-19. Microbiology Spectrum, 9(2), Article e00055-21. https://doi.org/10.1128/Spectrum.00055-21

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abstract = "Bacterial-viral interactions in saliva have been associated with morbidity and mortality for respiratory viruses such as influenza and SARS-CoV. However, such transkingdom relationships during SARS-CoV-2 infection are currently unknown. Here, we aimed to elucidate the relationship between saliva microbiota and SARSCoV- 2 in a cohort of newly hospitalized COVID-19 patients and controls. We used 16S rRNA sequencing to compare microbiome diversity and taxonomic composition between COVID-19 patients (n = 53) and controls (n = 59) and based on saliva SARSCoV- 2 viral load as measured using reverse transcription PCR (RT-PCR). The saliva microbiome did not differ markedly between COVID-19 patients and controls. However, we identified significant differential abundance of numerous taxa based on saliva SARS-CoV-2 viral load, including multiple species within Streptococcus and Prevotella.",

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doi = "10.1128/Spectrum.00055-21",

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AU - Nobel, Yael R.

AU - Soroush, Ali

AU - Blackett, John W.

AU - Krigel, Anna

AU - Phipps, Meaghan M.

AU - Freedberg, Daniel E.

AU - Zucker, Jason

AU - Sano, Ellen D.

AU - Uhlemann, Anne Catrin

AU - Abrams, Julian A.

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AB - Bacterial-viral interactions in saliva have been associated with morbidity and mortality for respiratory viruses such as influenza and SARS-CoV. However, such transkingdom relationships during SARS-CoV-2 infection are currently unknown. Here, we aimed to elucidate the relationship between saliva microbiota and SARSCoV- 2 in a cohort of newly hospitalized COVID-19 patients and controls. We used 16S rRNA sequencing to compare microbiome diversity and taxonomic composition between COVID-19 patients (n = 53) and controls (n = 59) and based on saliva SARSCoV- 2 viral load as measured using reverse transcription PCR (RT-PCR). The saliva microbiome did not differ markedly between COVID-19 patients and controls. However, we identified significant differential abundance of numerous taxa based on saliva SARS-CoV-2 viral load, including multiple species within Streptococcus and Prevotella.

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KW - SARS-CoV-2

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KW - Viral load

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Oral microbiome alterations and sars-cov-2 saliva viral load in patients with covid-19

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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