TY - JOUR
T1 - Optogenetic manipulation and photoacoustic imaging using a near-infrared transgenic mouse model
AU - Kasatkina, Ludmila A.
AU - Ma, Chenshuo
AU - Matlashov, Mikhail E.
AU - Vu, Tri
AU - Li, Mucong
AU - Kaberniuk, Andrii A.
AU - Yao, Junjie
AU - Verkhusha, Vladislav V.
N1 - Funding Information:
We thank H. Ai (University of Virginia) for the pBAD-AkaLuc plasmid, H. Shen (Duke University) for the animal surgery and care, M. Monakhov (Albert Einstein College of Medicine) for the initial characterization of the transgenic mouse, and the engineering team at Sonovol Inc. for the technical assistance with ultrasound imaging. Several figures were created with BioRender.com. This work was supported by the grants GM122567 (to V.V.V.), EB028143 (to J.Y.), NS111039 (to J.Y.) and NS115581 (to V.V.V. and J.Y.) from the US National Institutes of Health, 322226 from the Academy of Finland (to V.V.V.), and 226178 from the Chan Zuckerberg Initiative (to V.V.V. and J.Y.).
Funding Information:
We thank H. Ai (University of Virginia) for the pBAD-AkaLuc plasmid, H. Shen (Duke University) for the animal surgery and care, M. Monakhov (Albert Einstein College of Medicine) for the initial characterization of the transgenic mouse, and the engineering team at Sonovol Inc. for the technical assistance with ultrasound imaging. Several figures were created with BioRender.com. This work was supported by the grants GM122567 (to V.V.V.), EB028143 (to J.Y.), NS111039 (to J.Y.) and NS115581 (to V.V.V. and J.Y.) from the US National Institutes of Health, 322226 from the Academy of Finland (to V.V.V.), and 226178 from the Chan Zuckerberg Initiative (to V.V.V. and J.Y.).
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Optogenetic manipulation and optical imaging in the near-infrared range allow non-invasive light-control and readout of cellular and organismal processes in deep tissues in vivo. Here, we exploit the advantages of Rhodopseudomonas palustris BphP1 bacterial phytochrome, which incorporates biliverdin chromophore and reversibly photoswitches between the ground (740–800 nm) and activated (620–680 nm) states, to generate a loxP-BphP1 transgenic mouse model. The mouse enables Cre-dependent temporal and spatial targeting of BphP1 expression in vivo. We validate the optogenetic performance of endogenous BphP1, which in the activated state binds its engineered protein partner QPAS1, to trigger gene transcription in primary cells and living mice. We demonstrate photoacoustic tomography of BphP1 expression in different organs, developing embryos, virus-infected tissues and regenerating livers, with the centimeter penetration depth. The transgenic mouse model provides opportunities for both near-infrared optogenetics and photoacoustic imaging in vivo and serves as a source of primary cells and tissues with genomically encoded BphP1.
AB - Optogenetic manipulation and optical imaging in the near-infrared range allow non-invasive light-control and readout of cellular and organismal processes in deep tissues in vivo. Here, we exploit the advantages of Rhodopseudomonas palustris BphP1 bacterial phytochrome, which incorporates biliverdin chromophore and reversibly photoswitches between the ground (740–800 nm) and activated (620–680 nm) states, to generate a loxP-BphP1 transgenic mouse model. The mouse enables Cre-dependent temporal and spatial targeting of BphP1 expression in vivo. We validate the optogenetic performance of endogenous BphP1, which in the activated state binds its engineered protein partner QPAS1, to trigger gene transcription in primary cells and living mice. We demonstrate photoacoustic tomography of BphP1 expression in different organs, developing embryos, virus-infected tissues and regenerating livers, with the centimeter penetration depth. The transgenic mouse model provides opportunities for both near-infrared optogenetics and photoacoustic imaging in vivo and serves as a source of primary cells and tissues with genomically encoded BphP1.
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U2 - 10.1038/s41467-022-30547-6
DO - 10.1038/s41467-022-30547-6
M3 - Article
C2 - 35589810
AN - SCOPUS:85130325216
SN - 2041-1723
VL - 13
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 2813
ER -