Optical control of a rhodopsin-based switch

Ben Ovryn; Xiang Li; Hillel Chiel; Stefan Herlitze

doi:10.1117/12.578942

Optical control of a rhodopsin-based switch

Ben Ovryn, Xiang Li, Hillel Chiel, Stefan Herlitze

Anatomy & Structural Biology

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

A preliminary result supports the feasibility of using visible light to modulate the membrane potential of a cell. Human embryonic kidney cells (HEK293) were transfected with vertebrate rhodopsin and a gradient inward rectifying potassium (GIRK) channel. Whole cell patch clamp recordings of HEK293 cells exposed to 9-cis retinal showed that illumination increases the potassium current compared with recordings obtained in the dark. When combined with a rapid scanning device, this approach has the potential to control the activity of many neurons.

Original language	English (US)
Article number	49
Pages (from-to)	197-207
Number of pages	11
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	5
Issue number	13
DOIs	https://doi.org/10.1117/12.578942
State	Published - Dec 1 2004
Event	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI - San Jose, CA, United States Duration: Jan 27 2004 → Jan 29 2004

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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10.1117/12.578942

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AU - Li, Xiang

AU - Chiel, Hillel

AU - Herlitze, Stefan

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N2 - A preliminary result supports the feasibility of using visible light to modulate the membrane potential of a cell. Human embryonic kidney cells (HEK293) were transfected with vertebrate rhodopsin and a gradient inward rectifying potassium (GIRK) channel. Whole cell patch clamp recordings of HEK293 cells exposed to 9-cis retinal showed that illumination increases the potassium current compared with recordings obtained in the dark. When combined with a rapid scanning device, this approach has the potential to control the activity of many neurons.

AB - A preliminary result supports the feasibility of using visible light to modulate the membrane potential of a cell. Human embryonic kidney cells (HEK293) were transfected with vertebrate rhodopsin and a gradient inward rectifying potassium (GIRK) channel. Whole cell patch clamp recordings of HEK293 cells exposed to 9-cis retinal showed that illumination increases the potassium current compared with recordings obtained in the dark. When combined with a rapid scanning device, this approach has the potential to control the activity of many neurons.

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ER -

Optical control of a rhodopsin-based switch

Abstract

ASJC Scopus subject areas

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