Normal saline induces oxidative stress in peritoneal mesothelial cells

Alicja Połubinska, Andrzej Breborowicz, Ryszard Staniszewski, Dimitrios G. Oreopoulos

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Background: Peritoneal adhesions are the most common complication of the abdominal surgery. Normal saline is frequently used to rinse the peritoneal cavity during abdominal surgery, although there is no well-established data describing effect of such procedure on the process of formation of peritoneal adhesions. Methods: Effect of 0.9% NaCl solution on viability, oxidative stress, and fibrinolytic activity of human peritoneal mesothelial cells maintained in in vitro culture was evaluated. Results: Exposure of mesothelial cells to 0.9% NaCl induces oxidative stress, derangement of their structure with subsequent increased release of tissue factor (+75%) and plasminogen activator inhibitor-1 (+19%), and simultaneous suppression of tissue plasminogen activator release (-39%). In effect, ration tissue plasminogen activator/plasminogen activator inhibitor-1 was reduced in 0.9% NaCl-treated cells by 50%. Pretreatment of cells with precursor of glutathione synthesis: l-2-oxothiazolidine-4-carboxylic acid prevented these changes. Conclusions: Oxidative stress in the peritoneal mesothelium caused by 0.9% NaCl activates their procoagulant activity and impairs fibrinolytic properties of these cells. These effects disqualify 0.9% NaCl as rinsing solution during abdominal surgery.

Original languageEnglish (US)
Pages (from-to)1821-1826
Number of pages6
JournalJournal of Pediatric Surgery
Issue number10
StatePublished - Oct 2008
Externally publishedYes


  • Adhesions
  • Fibrinolytic activity
  • Mesothelium
  • Normal saline
  • Oxidative stress

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health


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