@article{2b3f1e97a7534ef4b3a7d2eaf9d21a9a,
title = "Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary",
abstract = "We report a method to identify neuropeptides in specific cell types, using mice with a conditional deletion of carboxypeptidase E. It was validated by deleting CPE in proopiomelanocortin cells. Surprisingly, these mice have greatly reduced levels of the anorexigenic peptide α-MSH but are not obese.",
keywords = "Proteomics, carboxypeptidase, neuropeptide, neuropeptidomics, peptidomics",
author = "Fricker, {Lloyd D.} and Tashima, {Alexandre K.} and Fakira, {Amanda K.} and Ute Hochgeschwender and Wetsel, {William C.} and Devi, {Lakshmi A.}",
note = "Funding Information: This work was supported by NIH grants DA008863 and NS026880 (to L.A.D.) and by Funda{\c c}{\~a}o de Amparo {\'a} Pesquisa do Estado de S{\~a}o Paulo , 2017/20106-9 , and Financiadora de Estudos e Projetos (to A.K.T.). The design of the CpeFlx was initiated in the Neurotransgenic Laboratory at Duke University Medical Center and supported by unrestricted funds (W.C.W.). The authors thank Adrei Jeltyi, Dylan Bitensky, and Dr. Lindsay Lueptow for assistance with the breeding of the mice, Erika S. Nishiduka for assistance with the liquid chromatography tandem mass spectrometry experiments, and Matthew Engel for assistance with peptidomic analysis. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",
year = "2021",
month = jan,
day = "21",
doi = "10.1016/j.chembiol.2020.11.003",
language = "English (US)",
volume = "28",
pages = "105--112.e4",
journal = "Cell Chemical Biology",
issn = "2451-9456",
publisher = "Elsevier Inc.",
number = "1",
}