Neuropeptide Y overexpression in the preweanling Zucker (fa/fa) rat

Timothy J. Kowalski, Thomas A. Houpt, Jeongwon Jahng, Nori Okada, Shun Mei Liu, Streamson C. Chua, Gerard P. Smith

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Hypothalamic preproNPY overexpression in the Zucker fatty (fa/fa) rat was examined. In situ hybridization was used to determine the relative level of preproNPY mRNA in the arcuate nucleus of +/+, +/fa, and fa/fa pups aged postnatal day 2 (P2), 5, 9, 12, or 25. The relative optical density (ROD) of probe hybridization in the arcuate, the area of hybridization (A), and the product of ROD x A (a measure of total arcuate preproNPY mRNA hybridization) were measured. Values were normalized to the mean +/fa value within each litter. Initial analysis showed that preproNPY mRNA hybridization (ROD x A) in fa/fa pups was significantly higher than +/fa and +/+ pups on P9, 12, and 25, and significantly higher than +/fa on P5. No significant difference between lean (+/+ and +/fa) genotypes, however, were observed at any age tested. Values from the lean genotypes were, therefore, pooled, and data were normalized to the mean value of lean animals for analysis. This analysis revealed that preproNPY mRNA hybridization in fa/fa pups was higher than lean littermates as early as P2. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)521-525
Number of pages5
JournalPhysiology and Behavior
Issue number4
StatePublished - Oct 1999
Externally publishedYes


  • Arcuate nucleus
  • Development of obesity
  • Genetic obesity
  • Hypothalamus

ASJC Scopus subject areas

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience


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