@inbook{c46e3389682243caa6d248a4e37a8287,
title = "Neuronal oxidative injury and biomarkers of lipid peroxidation",
abstract = "Oxidative stress is implicated as one of the major underlying mechanisms in a variety of human diseases. Reactive radicals, derived primarily from molecular oxygen, readily attack a variety of critical biological molecules, including DNA, cellular proteins, and lipids. Since lipid peroxidation is a central feature of cerebral oxidant injury, measurements of peroxidation products, such as F2-isoprostanes (F2-IsoPs) and F 4-neuroprostanes (F4-NeuroPs), represent the most accurate approaches to identify oxidative injury in vivo. Our laboratory uses a gas chromatography/mass spectrometry negative ion chemical ionization with select ion monitoring for quantification of these biomarkers of oxidative stress in a plethora of biological media. This review summarizes state-of-the-art methodology for F2-IsoPs and F4-NeuroPs analyses and discusses the utility of these prostaglandin-like compounds as in vivo biomarkers of neuronal oxidative injury.",
keywords = "F-isoprostanes, F-neuroprostanes, Lipid peroxidation, Neuronal tissue, Oxidative damage",
author = "Dejan Milatovic and Michael Aschner",
year = "2011",
doi = "10.1007/978-1-61779-077-5_17",
language = "English (US)",
isbn = "9781617790768",
series = "Neuromethods",
pages = "349--363",
editor = "Michael Aschner and Cristina Sunol and Anna Bal-Price",
booktitle = "Cell Culture Techniques",
}