TY - JOUR
T1 - Murine and human 3' IgH regulatory sequences
AU - Birshtein, B. K.
AU - Chen, C.
AU - Saleque, S.
AU - Michaelson, J. S.
AU - Singh, M.
AU - Little, R. D.
PY - 1997
Y1 - 1997
N2 - Both murine plasmacytomas and human Burkitt lymphomas contain chromosomal translocations involving the c-myc gene and the Ig gene cluster. As a result of the translocation in mouse plasmacytomas, the myc oncogene comes under the influence of regulatory sequences 3 of the murine IgH gene cluster. The murine 3 regulatory region contains four enhancers, three of which are located in a 25 kb unit of dyad symmetry [8, 14]. This unit contains a central enhancer, flanked by two identical enhancers at the termini, and we have speculated that this unit has entered the mouse genome during evolution as a transposable element. The fourth, and most downstream, enhancer comprises a separate structural and functional unit. Synergy among these enhancers has been documented and it has been suggested that these elements comprise a locus control region [6]. We have identified two virtually identical human enhancers 3 of the IgH locus, one downstream of the Cα1 gene and one downstream of the Cα2 gene. These bear a region of homology to one of the mouse 3 enhancers, and are predicted to play a role in myc ontogenesis. Preliminary evidence for DNA rearrangements of 3 regulatory sequences in human myeloma cell lines is presented.
AB - Both murine plasmacytomas and human Burkitt lymphomas contain chromosomal translocations involving the c-myc gene and the Ig gene cluster. As a result of the translocation in mouse plasmacytomas, the myc oncogene comes under the influence of regulatory sequences 3 of the murine IgH gene cluster. The murine 3 regulatory region contains four enhancers, three of which are located in a 25 kb unit of dyad symmetry [8, 14]. This unit contains a central enhancer, flanked by two identical enhancers at the termini, and we have speculated that this unit has entered the mouse genome during evolution as a transposable element. The fourth, and most downstream, enhancer comprises a separate structural and functional unit. Synergy among these enhancers has been documented and it has been suggested that these elements comprise a locus control region [6]. We have identified two virtually identical human enhancers 3 of the IgH locus, one downstream of the Cα1 gene and one downstream of the Cα2 gene. These bear a region of homology to one of the mouse 3 enhancers, and are predicted to play a role in myc ontogenesis. Preliminary evidence for DNA rearrangements of 3 regulatory sequences in human myeloma cell lines is presented.
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U2 - 10.1007/978-3-642-60801-8_7
DO - 10.1007/978-3-642-60801-8_7
M3 - Article
C2 - 9308230
AN - SCOPUS:0030863154
SN - 0070-217X
VL - 224
SP - 73
EP - 80
JO - Current Topics in Microbiology and Immunology
JF - Current Topics in Microbiology and Immunology
ER -