TY - JOUR
T1 - mSphere of Influence
T2 - Hooray for HaloTag! A novel tool to study autophagy in mammalian cells
AU - Cheney, Laura
N1 - Publisher Copyright:
Copyright © 2023 Cheney.
PY - 2023/12
Y1 - 2023/12
N2 - Laura Cheney works at the crossroads of HIV and autophagy, a critical biological process for cellular homeostasis, to understand more completely the pathogenesis of HIV-associated neurocognitive impairment. In this mSphere of Influence article, she reflects on how “A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag” by Willa Wen-You Yim, Hayashi Yamamoto, and Noboru Mizushima (eLife 11:e78923, 2022, https://doi.org/10.7554/eLife.78923) expands the tools for studying autophagy and inspired her to use this technology to develop a reporter to study autophagy of mitochondria, termed mitophagy, to further her own research goals.
AB - Laura Cheney works at the crossroads of HIV and autophagy, a critical biological process for cellular homeostasis, to understand more completely the pathogenesis of HIV-associated neurocognitive impairment. In this mSphere of Influence article, she reflects on how “A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag” by Willa Wen-You Yim, Hayashi Yamamoto, and Noboru Mizushima (eLife 11:e78923, 2022, https://doi.org/10.7554/eLife.78923) expands the tools for studying autophagy and inspired her to use this technology to develop a reporter to study autophagy of mitochondria, termed mitophagy, to further her own research goals.
KW - Atg8/LC3 processing assay
KW - HIV associated neurocognitive impairment
KW - HaloTag
KW - antiretroviral therapy
KW - autophagy
KW - mitophagy
UR - http://www.scopus.com/inward/record.url?scp=85180533906&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85180533906&partnerID=8YFLogxK
U2 - 10.1128/msphere.00482-23
DO - 10.1128/msphere.00482-23
M3 - Comment/debate
C2 - 37905922
AN - SCOPUS:85180533906
SN - 2379-5042
VL - 8
JO - mSphere
JF - mSphere
IS - 6
ER -
