Monitoring nanoparticle-mediated cellular hyperthermia with a high-sensitivity biosensor

Amarnath Mukherjee, Mark Castanares, Mohammad Hedayati, Michele Wabler, Bruce Trock, Prakash Kulkarni, Ronald Rodriguez, Robert H. Getzenberg, Theodore L. Deweese, Robert Ivkov, Shawn E. Lupold

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Aim: To develop and apply a heat-responsive and secreted reporter assay for comparing cellular response to nanoparticle (NP)- and macroscopic-mediated sublethal hyperthermia. Materials & methods: Reporter cells were heated by water bath (macroscopic heating) or iron oxide NPs activated by alternating magnetic fields (nanoscopic heating). Cellular responses to these thermal stresses were measured in the conditioned media by secreted luciferase assay. Results & conclusion: Reporter activity was responsive to macroscopic and nanoparticle heating and activity correlated with measured macroscopic thermal dose. Significant cellular responses were observed with NP heating under doses that were insufficient to measurably change the temperature of the system. Under these conditions, the reporter response correlated with proximity to cells loaded with heated nanoparticles. These results suggest that NP and macroscopic hyperthermia may be distinctive under conditions of mild hyperthermia. Original submitted 10 May 2013; Revised submitted 31 October 201.

Original languageEnglish (US)
Pages (from-to)2729-2743
Number of pages15
Issue number18
StatePublished - Dec 1 2014
Externally publishedYes


  • Metridia luciferase
  • heat shock
  • hyperthermia
  • iron oxide nanoparticle
  • nanoparticle

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)


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