A cDNA library has been constructed from adrenal glands of guinea pigs receiving a low sodium/high potassium diet. From this library a complementary DNA encoding an 11β-hydroxylase (EC 220.127.116.11) was isolated. Northern blot analysis revealed a major transcript of 2.2 kb size. The cDNA was cloned into an eucaryotic expression vector and the activity of the enzyme towards various substrates was investigated after expression in COS-1 cells. The enzyme was able to perform 11β-hydroxylations of 11-deoxycorticosterone, 11-deoxycortisol and androstendione as well as 18-hydroxylations and 19-hydroxylations to a small extent. Phylogenetic analyses with thirteen protein sequences of the CYP11B subfamily showed an early divergence of the guinea pig, but could not establish a definite branching order.
|Original language||English (US)|
|Number of pages||9|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Apr 16 1996|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology