Molecular cloning and expression of cDNA for mammalian translation initiation factor 5

Kallol Das; Jorge Chevesich; Umadas Maitra

doi:10.1073/pnas.90.7.3058

Molecular cloning and expression of cDNA for mammalian translation initiation factor 5

Kallol Das, Jorge Chevesich, Umadas Maitra

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S·AUG·Met-tRNA_f-eIF-2·GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated M_r 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.

Original language	English (US)
Pages (from-to)	3058-3062
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	7
DOIs	https://doi.org/10.1073/pnas.90.7.3058
State	Published - Apr 1 1993
Externally published	Yes

Keywords

GTP hydrolysis
GTPase superfamily proteins
Protein synthesis

ASJC Scopus subject areas

General

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10.1073/pnas.90.7.3058

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abstract = "Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S·AUG·Met-tRNAf-eIF-2·GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated Mr 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.",

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AU - Chevesich, Jorge

AU - Maitra, Umadas

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AB - Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S·AUG·Met-tRNAf-eIF-2·GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated Mr 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.

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KW - Protein synthesis

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Molecular cloning and expression of cDNA for mammalian translation initiation factor 5

Abstract

Keywords

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