Modified microkeratome-assisted posterior lamellar keratoplasty using a tissue adhesive

Ashkan Pirouzmanesh, Samantha Herretes, Johann M.G. Reyes, Olan Suwan-apichon, Roy S. Chuck, Dong An Wang, Jennifer H. Elisseeff, Walter J. Stark, Ashley Behrens

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Objective: To compare graft stability and astigmatic change using suture vs tissue adhesive in an experimental model of microkeratome-assisted posterior lamellar keratoplasty. Methods: A 300-μm-thick partial flap keratectomy was performed in human donor corneoscleral rims using an artificial anterior chamber and a manual microkeratome. The flap stopped at the left central opening border, providing a wide hinge to add stability. After flap reflection, a 6.25-mm trephination was performed to obtain a disc of posterior stroma, Descemet membrane, and endothelium. The disc was positioned in a sutureless fashion, and the flap secured with either 5 interrupted sutures or a chondroitin-sulfate-aldehyde-based adhesive. Increasing intrachamber pressures were created to detect graft stability. Videokeratographic data were recorded to evaluate astigmatic change. Results: The mean (SD) astigmatic change was 3.08 (0.84) diopters (D) in the sutured group and 1.13 (0.55) D in the glued group (P = .008). Mean (SD) resisted pressures were 95.68 (27.38) mm Hg and 82.45 (18.40) mm Hg in the sutured and glued groups, respectively (P = .97). Conclusion: This modified technique of microkeratome-assisted posterior lamellar keratoplasty showed excellent graft stability in both groups. Flaps sealed with the novel tissue adhesive had reduced astigmatic changes in our experimental model. Clinical Relevance: Sutureless microkeratome-assisted posterior lamellar keratoplasty using tissue adhesive may become a new alternative in the surgical treatment of corneal endothelial disorders.

Original languageEnglish (US)
Pages (from-to)210-214
Number of pages5
JournalArchives of Ophthalmology
Issue number2
StatePublished - Feb 2006
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology


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