Mode of action of colicin Ib Formation of ion-permeable membrane channels

Craig A. Weaver, Bruce L. Kagan, Alan Finkelstein, Jordan Konisky

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Addition of purified colicin Ib to whole Escherichia coli cells or cytoplasmic membrane vesicles inhibits their subsequent ability to generate a membrane potential. In addition, this colicin is shown to bring about a voltage-dependent increase in the conductance of an artificial planar bilayer membrane prepared from soybean phospholipids. This results from the formation of ion-permeable channels. These data provide strong evidence that the depolarization of Escherichia coli cells by this colicin results from an Ib-induced increase in membrane permeability to ions.

Original languageEnglish (US)
Pages (from-to)137-142
Number of pages6
JournalBBA - Biomembranes
Issue number1
StatePublished - Jun 6 1981


  • (E. coli, Planar bilayer, Vesicle)
  • Colicin Ib
  • Ion channel
  • Ion permeability
  • Membrane potential

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


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