Microtubule arrays in taxol-treated mouse dorsal root ganglion-spinal cord cultures

Edmund B. Masurovsky; Edith R. Peterson; Stanley M. Crain; Susan Band Horwitz

doi:10.1016/0006-8993(81)90017-2

Microtubule arrays in taxol-treated mouse dorsal root ganglion-spinal cord cultures

Edmund B. Masurovsky, Edith R. Peterson, Stanley M. Crain, Susan Band Horwitz

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

Exposure of organotypic dorsal root ganglion-spinal cord cultures to taxol, a potent microtubule promoting and stabilizing agent, results in an unusual abundance of microtubules in neurons, and the presence of microtubule-endoplasmic reticulum arrays in their perikarya and processes. Ordered concentric arrays of microtubules alternating with marcromolecular material are observed in dorsal root ganglion neurites. Short linear structures are discernible between some microtubules and the macromolecular material, as well as between microtubules and endoplasmic reticulum cisternae. Analyses of such unusual microtuble arrays in taxol-treated cultures may provide valuable insights into tubulin-related systems in neurons, as well as in other cells.

Original language	English (US)
Pages (from-to)	392-398
Number of pages	7
Journal	Brain research
Volume	217
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(81)90017-2
State	Published - Aug 3 1981

Keywords

CNS neurons
dorsal root ganglion neurons
microtubule arrays
microtubules
nerve tissue culture
taxol

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(81)90017-2

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@article{59b3c05470f74c208b57beab88a817a9,

title = "Microtubule arrays in taxol-treated mouse dorsal root ganglion-spinal cord cultures",

abstract = "Exposure of organotypic dorsal root ganglion-spinal cord cultures to taxol, a potent microtubule promoting and stabilizing agent, results in an unusual abundance of microtubules in neurons, and the presence of microtubule-endoplasmic reticulum arrays in their perikarya and processes. Ordered concentric arrays of microtubules alternating with marcromolecular material are observed in dorsal root ganglion neurites. Short linear structures are discernible between some microtubules and the macromolecular material, as well as between microtubules and endoplasmic reticulum cisternae. Analyses of such unusual microtuble arrays in taxol-treated cultures may provide valuable insights into tubulin-related systems in neurons, as well as in other cells.",

keywords = "CNS neurons, dorsal root ganglion neurons, microtubule arrays, microtubules, nerve tissue culture, taxol",

author = "Masurovsky, {Edmund B.} and Peterson, {Edith R.} and Crain, {Stanley M.} and Horwitz, {Susan Band}",

note = "Funding Information: This work was supported by NINCDS research Grant NS 14990 to SMC, and by the American Cancer Society Grant CH-86 to SBH. We thank Drs. Pat Model and Peter Schiff for helpful discussions. Electron microscopy and related facilities were kindly provided by Dr. Pat Model and tissue culture facilities by Dr. Murray Bornstein.",

year = "1981",

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doi = "10.1016/0006-8993(81)90017-2",

language = "English (US)",

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pages = "392--398",

journal = "Brain research",

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T1 - Microtubule arrays in taxol-treated mouse dorsal root ganglion-spinal cord cultures

AU - Masurovsky, Edmund B.

AU - Peterson, Edith R.

AU - Crain, Stanley M.

AU - Horwitz, Susan Band

N1 - Funding Information: This work was supported by NINCDS research Grant NS 14990 to SMC, and by the American Cancer Society Grant CH-86 to SBH. We thank Drs. Pat Model and Peter Schiff for helpful discussions. Electron microscopy and related facilities were kindly provided by Dr. Pat Model and tissue culture facilities by Dr. Murray Bornstein.

PY - 1981/8/3

Y1 - 1981/8/3

N2 - Exposure of organotypic dorsal root ganglion-spinal cord cultures to taxol, a potent microtubule promoting and stabilizing agent, results in an unusual abundance of microtubules in neurons, and the presence of microtubule-endoplasmic reticulum arrays in their perikarya and processes. Ordered concentric arrays of microtubules alternating with marcromolecular material are observed in dorsal root ganglion neurites. Short linear structures are discernible between some microtubules and the macromolecular material, as well as between microtubules and endoplasmic reticulum cisternae. Analyses of such unusual microtuble arrays in taxol-treated cultures may provide valuable insights into tubulin-related systems in neurons, as well as in other cells.

AB - Exposure of organotypic dorsal root ganglion-spinal cord cultures to taxol, a potent microtubule promoting and stabilizing agent, results in an unusual abundance of microtubules in neurons, and the presence of microtubule-endoplasmic reticulum arrays in their perikarya and processes. Ordered concentric arrays of microtubules alternating with marcromolecular material are observed in dorsal root ganglion neurites. Short linear structures are discernible between some microtubules and the macromolecular material, as well as between microtubules and endoplasmic reticulum cisternae. Analyses of such unusual microtuble arrays in taxol-treated cultures may provide valuable insights into tubulin-related systems in neurons, as well as in other cells.

KW - CNS neurons

KW - dorsal root ganglion neurons

KW - microtubule arrays

KW - microtubules

KW - nerve tissue culture

KW - taxol

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U2 - 10.1016/0006-8993(81)90017-2

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AN - SCOPUS:0019496291

SN - 0006-8993

VL - 217

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EP - 398

JO - Brain research

JF - Brain research

IS - 2

ER -

Microtubule arrays in taxol-treated mouse dorsal root ganglion-spinal cord cultures

Abstract

Keywords

ASJC Scopus subject areas

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