@inbook{139e5ad347674454b23978170342e55a,
title = "Methods to study monocyte migration induced by HIV-infected cells",
abstract = "HIV-associated dementia (HAD) is a multi-factorial disease set in motion by the presence of HIV-infected cells in the brain. A characteristic feature of HAD is the infiltration of mononuclear phagocytes into the brain, which is aided by HIV-1 Tat protein and other chemokines secreted by both HIV-infected cells and uninfected cells in their vicinity. Both direct and indirect chemokine activity of HIV-1 Tat protein has been demonstrated employing purified recombinant Tat protein. However, a corroboration of a key role for Tat or other chemokines in monocyte migration, in the context of HIV-infection, has not yet been demonstrated. Here we describe methods, to measure the role of soluble factors, such as chemokines and Tat, released by HIV-infected cells or uninfected cells in their vicinity, in monocyte migration in vitro.",
keywords = "HIV-associated dementia/monocyte migration/monocyte chemotaxis/HIV-1 Tat/monocyte chemotaxis protein-1/chemokines",
author = "Rao, {Vasudev R.} and Eugenin, {Eliseo A.} and Berman, {Joan W.} and Prasad, {Vinayaka R.}",
year = "2009",
doi = "10.1007/978-1-59745-170-3_20",
language = "English (US)",
isbn = "9781588298591",
series = "Methods in Molecular Biology",
pages = "295--309",
editor = "Prasad, {Vinayaka R.} and Kalpana, {Ganjam V.}",
booktitle = "HIV Protocols",
}
