TY - JOUR
T1 - Methodologies to decipher the cell secretome
AU - Mukherjee, Paromita
AU - Mani, Sridhar
N1 - Funding Information:
The work of the laboratory is supported by the National Institutes of Health grant RO1CA127231 and RO1CA161879 and Albert Einstein College of medicine.
PY - 2013
Y1 - 2013
N2 - The cell secretome is a collection of proteins consisting of transmembrane proteins (TM) and proteins secreted by cells into the extracellular space. A significant portion (~ 13-20%) of the human proteome consists of secretory proteins. The secretory proteins play important roles in cell migration, cell signaling and communication. There is a plethora of methodologies available like Serial Analysis of Gene Expression (SAGE), DNA microarrays, antibody arrays and bead-based arrays, mass spectrometry, RNA sequencing and yeast, bacterial and mammalian secretion traps to identify the cell secretomes. There are many advantages and disadvantages in using any of the above methods. This review aims to discuss the methodologies available along with their potential advantages and disadvantages to identify secretory proteins. This review is a part of a Special issue on The Secretome. This article is part of a Special Issue entitled: An Updated Secretome.
AB - The cell secretome is a collection of proteins consisting of transmembrane proteins (TM) and proteins secreted by cells into the extracellular space. A significant portion (~ 13-20%) of the human proteome consists of secretory proteins. The secretory proteins play important roles in cell migration, cell signaling and communication. There is a plethora of methodologies available like Serial Analysis of Gene Expression (SAGE), DNA microarrays, antibody arrays and bead-based arrays, mass spectrometry, RNA sequencing and yeast, bacterial and mammalian secretion traps to identify the cell secretomes. There are many advantages and disadvantages in using any of the above methods. This review aims to discuss the methodologies available along with their potential advantages and disadvantages to identify secretory proteins. This review is a part of a Special issue on The Secretome. This article is part of a Special Issue entitled: An Updated Secretome.
KW - Secretome
KW - Secretory protein
KW - Signal peptides
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U2 - 10.1016/j.bbapap.2013.01.022
DO - 10.1016/j.bbapap.2013.01.022
M3 - Review article
C2 - 23376189
AN - SCOPUS:84884819754
SN - 1570-9639
VL - 1834
SP - 2226
EP - 2232
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
IS - 11
ER -