Mechanism and regulation of mycobactin fatty acyl-AMP ligase FadD33

Olivia Vergnolle, Hua Xu, John S. Blanchard

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Background: Fatty acyl-AMP ligase FadD33 is required for mycobactin biosynthesis. Results: FadD33 catalyzes a two-step kinetic mechanism, and FadD33 activity is regulated by post-translational acetylation. Conclusion: Mycobactin FadD33 activity is reversibly regulated by Pat (acetylation) and DAc1 (deacetylation). Significant: Post-translational regulation via acetylation of enzymes can modulate siderophore biosynthesis.

Original languageEnglish (US)
Pages (from-to)28116-28125
Number of pages10
JournalJournal of Biological Chemistry
Issue number39
StatePublished - Sep 27 2013

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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