Mapping T cell epitopes by flow cytometry

Bodo Hoffmeister, Felix Kiecker, Lydia Tesfa, Hans Dieter Volk, Louis J. Picker, Florian Kern

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


Epitope mapping by flow cytometry is a very modern approach that not only identifies T-cell epitopes but simultaneously allows for detailed analysis of the responding T-cell subsets including lineage, activation marker expression, and other markers of interest. The most frequently used approach is based on the identification of intracellular cytokines in secretion-inhibited activated T cells following stimulation with peptides or peptide pools. A more recently developed assay analyzes T-cell proliferation by measuring the decrease in carboxyfluorescein diacetate succinimidyl ester staining in proliferated cells. This article includes information on peptide configuration, a section on the design and efficient application of peptide pools, and working laboratory protocols for both assays.

Original languageEnglish (US)
Pages (from-to)270-281
Number of pages12
Issue number3
StatePublished - Mar 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology


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