TY - JOUR
T1 - Mapping of Brain Activity by Automated Volume Analysis of Immediate Early Genes
AU - Renier, Nicolas
AU - Adams, Eliza L.
AU - Kirst, Christoph
AU - Wu, Zhuhao
AU - Azevedo, Ricardo
AU - Kohl, Johannes
AU - Autry, Anita E.
AU - Kadiri, Lolahon
AU - Umadevi Venkataraju, Kannan
AU - Zhou, Yu
AU - Wang, Victoria X.
AU - Tang, Cheuk Y.
AU - Olsen, Olav
AU - Dulac, Catherine
AU - Osten, Pavel
AU - Tessier-Lavigne, Marc
N1 - Funding Information:
J.K. is supported by a Human Frontier Long-Term Fellowship, an EMBO Long-Term Fellowship and a Sir Henry Wellcome Fellowship. A.E.A. is supported by the Eunice Kennedy Shriver National Institute of Child Health and Development of the NIH under award F32HD078040 and by a NARSAD Young Investigator award. This work was supported by NIH grant 1R01HD082131-01A1 (to C.D.) and by the Rockefeller University (to M.T.-L.). C.D. is an investigator of the Howard Hughes Medical Institute.
Funding Information:
We would like to thank Nils Brose for helpful discussions. We are also grateful to Yingxi Lin for sharing reagents and insights. We would also like to thank members of the M.T.-L. lab for helpful discussions. Our gratitude goes to the Rockefeller University Bio-Imaging Resource Center, and, in particular, Pablo Ariel, Kaye Thomas, Tao Tong, and Alison North. N.R. is supported by a Shelby White and Leon Levy fellowship. E.L.A. is supported by a fellowship from the Rockefeller University Women & Science Initiative. J.K. is supported by a Human Frontier Long-Term Fellowship, an EMBO Long-Term Fellowship and a Sir Henry Wellcome Fellowship. A.E.A. is supported by the Eunice Kennedy Shriver National Institute of Child Health and Development of the NIH under award F32HD078040 and by a NARSAD Young Investigator award. This work was supported by NIH grant 1R01HD082131-01A1 (to C.D.) and by the Rockefeller University (to M.T.-L.). C.D. is an investigator of the Howard Hughes Medical Institute.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/6/16
Y1 - 2016/6/16
N2 - Understanding how neural information is processed in physiological and pathological states would benefit from precise detection, localization, and quantification of the activity of all neurons across the entire brain, which has not, to date, been achieved in the mammalian brain. We introduce a pipeline for high-speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program we term ClearMap. We validate the pipeline first by analysis of brain regions activated in response to haloperidol. Next, we report new cortical regions downstream of whisker-evoked sensory processing during active exploration. Last, we combine activity mapping with axon tracing to uncover new brain regions differentially activated during parenting behavior. This pipeline is widely applicable to different experimental paradigms, including animal species for which transgenic activity reporters are not readily available.
AB - Understanding how neural information is processed in physiological and pathological states would benefit from precise detection, localization, and quantification of the activity of all neurons across the entire brain, which has not, to date, been achieved in the mammalian brain. We introduce a pipeline for high-speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program we term ClearMap. We validate the pipeline first by analysis of brain regions activated in response to haloperidol. Next, we report new cortical regions downstream of whisker-evoked sensory processing during active exploration. Last, we combine activity mapping with axon tracing to uncover new brain regions differentially activated during parenting behavior. This pipeline is widely applicable to different experimental paradigms, including animal species for which transgenic activity reporters are not readily available.
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U2 - 10.1016/j.cell.2016.05.007
DO - 10.1016/j.cell.2016.05.007
M3 - Article
C2 - 27238021
AN - SCOPUS:84973441068
SN - 0092-8674
VL - 165
SP - 1789
EP - 1802
JO - Cell
JF - Cell
IS - 7
ER -