TY - JOUR
T1 - Maintenance of mouse, rat, and pig pancreatic islet functions by coculture with human islet-derived fibroblasts
AU - Miki, Atsushi
AU - Narushima, Michiki
AU - Okitsu, Teru
AU - Takeno, Yuichi
AU - Soto-Gutierrez, Alejandro
AU - Rivas-Carrillo, Jorge David
AU - Navarro-Alvarez, Nalú
AU - Chen, Yong
AU - Tanaka, Kimiaki
AU - Noguchi, Hirofumi
AU - Matsumoto, Shinichi
AU - Kohara, Michinori
AU - Lakey, Jonathan R.T.
AU - Kobayashi, Eiji
AU - Tanaka, Noriaki
AU - Kobayashi, Naoya
PY - 2006
Y1 - 2006
N2 - Development of an efficient preculture system of islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for a source as a coculture system for freshly isolated islets to maintain islet functions. Human pancreatic islet cells were nucleofected with a plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance gene (HygroR). One of the transduced cell lines, MNNK-1, was established and served as a feeder cell in the coculture for freshly isolated mouse, rat, and pig islets. Morphology, viability, and glucose-responding insulin secretion were analyzed in the coculture system. MNNK-1 cells were morphologically spindle shaped and were negative for pancreatic endocrine markers. MNNK-1 cells were positive for α-smooth muscle actin and collagen type I and produced fibroblast growth factor. Coculture of the mouse, rat, and pig islets with MNNK-1 cells maintained their viability and insulin secretion with glucose responsiveness. A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system.
AB - Development of an efficient preculture system of islets is ideal. Toward that goal, we constructed a human pancreatic islet-derived fibroblast cell line MNNK-1 for a source as a coculture system for freshly isolated islets to maintain islet functions. Human pancreatic islet cells were nucleofected with a plasmid vector pYK-1 expressing simian virus 40 large T antigen gene (SV40T) and hygromycin resistance gene (HygroR). One of the transduced cell lines, MNNK-1, was established and served as a feeder cell in the coculture for freshly isolated mouse, rat, and pig islets. Morphology, viability, and glucose-responding insulin secretion were analyzed in the coculture system. MNNK-1 cells were morphologically spindle shaped and were negative for pancreatic endocrine markers. MNNK-1 cells were positive for α-smooth muscle actin and collagen type I and produced fibroblast growth factor. Coculture of the mouse, rat, and pig islets with MNNK-1 cells maintained their viability and insulin secretion with glucose responsiveness. A human pancreatic islet-derived fibroblast cell line MNNK-1 was established. MNNK-1 cells were a useful means for maintaining morphology and insulin secretion of islets in the coculture system.
KW - Coculture
KW - Islets
KW - Simian virus 40 large T antigen
UR - http://www.scopus.com/inward/record.url?scp=33746728234&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746728234&partnerID=8YFLogxK
U2 - 10.3727/000000006783981882
DO - 10.3727/000000006783981882
M3 - Article
C2 - 16898226
AN - SCOPUS:33746728234
SN - 0963-6897
VL - 15
SP - 325
EP - 334
JO - Cell Transplantation
JF - Cell Transplantation
IS - 4
ER -