TY - JOUR
T1 - Low molecular weight iron from guinea pig reticulocytes isolated by Sephadex G‐25 chromatography
AU - Weaver, Janet
AU - Pollack, Simeon
AU - Zhan, Hui
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1989/10
Y1 - 1989/10
N2 - As part of a continuing study of the low MW iron pool, guinea pig reticulocytes were incubated with 59Fe‐labeled transferrin, and the reticulocyte hemolysate was chromatographed on Sephadex G‐25. 59Fe, in amounts corresponding to that which was in a low MW peak eluting from an Ultrogel column and to that not precipitated by ammonium sulfate, adsorbed to the Sephadex column. The adsorbing 59Fe, on elution from the Sephadex with dilute formic acid, coeluted with phosphate and pentose. When EDTA was added to disrupt the putative iron complex, neither iron nor P adsorbed to the column, supporting the argument that they exist as a compound in the cytosol and adsorb and elute together for that reason. These observations provide additional evidence that P‐containing compounds, probably originating as nucleotides, are important components of the low MW iron pool of cells.
AB - As part of a continuing study of the low MW iron pool, guinea pig reticulocytes were incubated with 59Fe‐labeled transferrin, and the reticulocyte hemolysate was chromatographed on Sephadex G‐25. 59Fe, in amounts corresponding to that which was in a low MW peak eluting from an Ultrogel column and to that not precipitated by ammonium sulfate, adsorbed to the Sephadex column. The adsorbing 59Fe, on elution from the Sephadex with dilute formic acid, coeluted with phosphate and pentose. When EDTA was added to disrupt the putative iron complex, neither iron nor P adsorbed to the column, supporting the argument that they exist as a compound in the cytosol and adsorb and elute together for that reason. These observations provide additional evidence that P‐containing compounds, probably originating as nucleotides, are important components of the low MW iron pool of cells.
KW - iron
KW - reticulocyte
UR - http://www.scopus.com/inward/record.url?scp=0024807199&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024807199&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0609.1989.tb00306.x
DO - 10.1111/j.1600-0609.1989.tb00306.x
M3 - Article
C2 - 2511038
AN - SCOPUS:0024807199
SN - 0902-4441
VL - 43
SP - 321
EP - 327
JO - European Journal of Haematology
JF - European Journal of Haematology
IS - 4
ER -