Live cell imaging of RhoGTPase biosensors in tumor cells

Jose Javier Bravo-Cordero, Yasmin Moshfegh, John Condeelis, Louis Hodgson

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations


Tumor cell motility and invasion rely on actin cytoskeleton rearrangements mediated by the activation of RhoGTPase signaling pathways. Invadopodia are membrane-degrading protrusions that mediate extracellular matrix degradation. Here, we provide procedures for imaging RhoGTPase biosensors in tumor cells during the formation of invadopodia and matrix degradation.

Original languageEnglish (US)
Title of host publicationAdhesion Protein Protocols
PublisherHumana Press Inc.
Number of pages12
ISBN (Print)9781627035378
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Biosensors
  • FRET
  • Invadopodia
  • RhoGTPase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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