@inbook{3849cd4b8e78477495369b7fb55fa6e9,
title = "Live-cell Imaging of Biosynthetic Protein Transport in Hepatocytes",
abstract = "Here, we describe a strategy to analyze the exit of apical and basolateral cargo from the trans-Golgi network in primary hepatocytes. The method is based on recombinant adenovirus-mediated infection combined with a pulse-chase regimen and live-cell imaging analysis of fluorescent protein-tagged dipeptidyl peptidase IV (DPPIV) and vesicular stomatitis virus G (VSVG) cargo proteins, coexpressed and accumulated in the endoplasmic reticulum via DPPIV aggregation through an engineered conditional aggregation domain and VSVG by exploiting the aggregation of the ts045 mutant at its non-permissive temperature of 40 °C.",
keywords = "Apical cargo, Conditional aggregation domain, Hepatocyte, Live-cell imaging, Protein sorting, Protein targeting, Protein transport, trans-Golgi network",
author = "Francisco L{\'a}zaro-Di{\'e}guez and Anne M{\"u}sch",
note = "Funding Information: We acknowledge David S. Neufeld and Fadi-Luc Jaber at the Albert Einstein COM for performing rat liver perfusions. David Cohen from our group for technical advice and preparations of the adenoviruses. This work was supported by the National Institutes of Health grant R01 DK118015 and institutional funds from Albert Einstein College of Medicine to AM. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2557-6_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "145--157",
booktitle = "Methods in Molecular Biology",
}