TY - JOUR
T1 - Lipid droplet levels vary heterogeneously in response to simulated gastrointestinal stresses in different probiotic Saccharomyces cerevisiae strains
AU - Zamith-Miranda, Daniel
AU - Palma, Mariana L.
AU - Matos, Gabriel S.
AU - Schiebel, Johnathon G.
AU - Maya-Monteiro, Clarissa M.
AU - Aronovich, Marcos
AU - Bozza, Patricia T.
AU - Bozza, Fernando A.
AU - Nimrichter, Leonardo
AU - Montero-Lomeli, Monica
AU - Marques, Ernesto T.A.
AU - Martins, Flaviano S.
AU - Douradinha, Bruno
N1 - Funding Information:
We thank Professor Cláudio A. Masuda from Universidade Federal do Rio de Janeiro, Brazil, for critically reviewing this manuscript and helpful scientific discussions, Stefan Lamers for manuscript editing and Jens Rietdorf (CAPES/CTDS) for technical assistance. This work was supported by grants from Conselho Nacional de Ciência e Tecnologia (CNPq; Brasília, DF, Brazil), Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ, Rio de Janeiro, RJ, Brazil), Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG, Belo Horizonte, MG, Brazil) and Fondazione RiMED (Palermo, PA, Italy). MM-L is the recipient of a FAPERJ Cientistas do Nosso Estado grant. GSM is the recipient of a CNPq Iniciação Científica fellowship. FSM is the recipient of a CNPq research fellowship. BD is a Fondazione RiMED Associate Scholar. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - To exert their therapeutic action, probiotic Saccharomyces cerevisiae strains must survive harsh digestive environments. Lipid droplets accumulate in cells which undergo stress-inducing situations, supposedly having a protective role. We assessed lipid droplet levels, either naturally accumulated or induced in response to digestive challenges, of probiotic strains S. boulardii, S. cerevisiae A-905, S. cerevisiae Sc47 and S. cerevisiae L11, and of non-probiotic strains S. cerevisiae BY4741 and S. cerevisiae BY4743. Strains 905 and Sc47 had lower and higher lipid droplet levels, respectively, when compared to the remaining strains, showing that higher accumulationof these neutral lipids is not a feature shared by all probiotic Saccharomyces strains. When submitted to simulated gastric or bile salts environments, lipid droplet levels increase in all tested probiotic strains, at least for one to the induced stresses, suggesting that lipid droplets participate in the protective mechanisms against gastrointestinal stresses in probiotic Saccharomyces yeasts.
AB - To exert their therapeutic action, probiotic Saccharomyces cerevisiae strains must survive harsh digestive environments. Lipid droplets accumulate in cells which undergo stress-inducing situations, supposedly having a protective role. We assessed lipid droplet levels, either naturally accumulated or induced in response to digestive challenges, of probiotic strains S. boulardii, S. cerevisiae A-905, S. cerevisiae Sc47 and S. cerevisiae L11, and of non-probiotic strains S. cerevisiae BY4741 and S. cerevisiae BY4743. Strains 905 and Sc47 had lower and higher lipid droplet levels, respectively, when compared to the remaining strains, showing that higher accumulationof these neutral lipids is not a feature shared by all probiotic Saccharomyces strains. When submitted to simulated gastric or bile salts environments, lipid droplet levels increase in all tested probiotic strains, at least for one to the induced stresses, suggesting that lipid droplets participate in the protective mechanisms against gastrointestinal stresses in probiotic Saccharomyces yeasts.
KW - Gastrointestinal tract stresses
KW - Lipid droplets
KW - Probiotics
KW - Saccharomyces cerevisiae
KW - Saccharomyces cerevisiae var boulardii
KW - Stress resistance
UR - http://www.scopus.com/inward/record.url?scp=84957881788&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957881788&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2015.12.013
DO - 10.1016/j.jff.2015.12.013
M3 - Article
AN - SCOPUS:84957881788
SN - 1756-4646
VL - 21
SP - 193
EP - 200
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -