Linkage analysis by two-dimensional DNA typing

G. J. Te Meerman, E. Mullaart, M. A. Van der Meulen, J. H.G. Den Daas, B. Morolli, A. G. Uitterlinden, J. Vijg

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


In two-dimensional (2-D) DNA typing, genomic DNA fragments are separated, first according to size by electrophoresis in a neutral polyacrylamide gel and second according to sequence by denaturing gradient gel electrophoresis, followed by hybridization analysis using micro- and minisatellite core probes. The 2-D DNA typing method generates a large amount of information on polymorphic loci per gel. Here we demonstrate the potential usefulness of 2- D DNA typing in an empirical linkage study on the red factor in cattle, and we show an example of the 2-D DNA typing analysis of a human pedigree. The power efficiency of 2-D DNA typing in general is compared with that of single-locus typing by simulation. The results indicate that, although 2-D DNA typing is very efficient in generating data on polymorphic loci, its power to detect linkage is lower than single-locus typing, because it is not obvious whether a spot represents the presence of one or two alleles. It is possible to compensate for this lower informativeness by increasing the sample size. Genome scanning by 2-D DNA typing has the potential to be more efficient than current genotyping methods in scoring polymorphic loci. Hence, it could become a method of choice in mapping genetic traits in humans and animals.

Original languageEnglish (US)
Pages (from-to)1289-1297
Number of pages9
JournalAmerican Journal of Human Genetics
Issue number6
StatePublished - Jan 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)


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