LINE-1 activity in facultative heterochromatin formation during X chromosome inactivation

Jennifer C. Chow; Constance Ciaudo; Melissa J. Fazzari; Nathan Mise; Nicolas Servant; Jacob L. Glass; Matthew Attreed; Philip Avner; Anton Wutz; Emmanuel Barillot; John M. Greally; Olivier Voinnet; Edith Heard

doi:10.1016/j.cell.2010.04.042

LINE-1 activity in facultative heterochromatin formation during X chromosome inactivation

Jennifer C. Chow, Constance Ciaudo, Melissa J. Fazzari, Nathan Mise, Nicolas Servant, Jacob L. Glass, Matthew Attreed, Philip Avner, Anton Wutz, Emmanuel Barillot, John M. Greally, Olivier Voinnet, Edith Heard

Research output: Contribution to journal › Article › peer-review

249 Scopus citations

Abstract

During X chromosome inactivation (XCI), Xist RNA coats and silences one of the two X chromosomes in female cells. Little is known about how XCI spreads across the chromosome, although LINE-1 elements have been proposed to play a role. Here we show that LINEs participate in creating a silent nuclear compartment into which genes become recruited. A subset of young LINE-1 elements, however, is expressed during XCI, rather than being silenced. We demonstrate that such LINE expression requires the specific heterochromatic state induced by Xist. These LINEs often lie within escape-prone regions of the X chromosome, but close to genes that are subject to XCI, and are associated with putative endo-siRNAs. LINEs may thus facilitate XCI at different levels, with silent LINEs participating in assembly of a heterochromatic nuclear compartment induced by Xist, and active LINEs participating in local propagation of XCI into regions that would otherwise be prone to escape. PaperClip:

Original language	English (US)
Pages (from-to)	956-969
Number of pages	14
Journal	Cell
Volume	141
Issue number	6
DOIs	https://doi.org/10.1016/j.cell.2010.04.042
State	Published - Jun 2010

Keywords

DNA
Devbio
RNA

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2010.04.042

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@article{b67b7fd7b3f04564a4a535034f9783c7,

title = "LINE-1 activity in facultative heterochromatin formation during X chromosome inactivation",

abstract = "During X chromosome inactivation (XCI), Xist RNA coats and silences one of the two X chromosomes in female cells. Little is known about how XCI spreads across the chromosome, although LINE-1 elements have been proposed to play a role. Here we show that LINEs participate in creating a silent nuclear compartment into which genes become recruited. A subset of young LINE-1 elements, however, is expressed during XCI, rather than being silenced. We demonstrate that such LINE expression requires the specific heterochromatic state induced by Xist. These LINEs often lie within escape-prone regions of the X chromosome, but close to genes that are subject to XCI, and are associated with putative endo-siRNAs. LINEs may thus facilitate XCI at different levels, with silent LINEs participating in assembly of a heterochromatic nuclear compartment induced by Xist, and active LINEs participating in local propagation of XCI into regions that would otherwise be prone to escape. PaperClip:",

keywords = "DNA, Devbio, RNA",

author = "Chow, {Jennifer C.} and Constance Ciaudo and Fazzari, {Melissa J.} and Nathan Mise and Nicolas Servant and Glass, {Jacob L.} and Matthew Attreed and Philip Avner and Anton Wutz and Emmanuel Barillot and Greally, {John M.} and Olivier Voinnet and Edith Heard",

note = "Funding Information: We would like to thank members of the Heard laboratory and Vincent Colot for critical reading of the manuscript; John Goodier and Deborah Bourc'his for advice and mouse LINE-1 plasmid constructs; and Valerie Cognat (IBMP) for expert bioinformatics analyses. This work was supported by grants from the ANR (“RNA ES”; to both E.H. and O.V.); FRM (Equipe FRM to E.H. and FRM fellowship to J.C.); HEROIC, an Integrated Project funded by the European Union under the 6th Framework Programme (LSHG-CT-2005-018883); and Canceropole IdF (GET project) to E.H; O.V.'s laboratory was further supported by an ERC Young Investigator Award (Grant ERC-210890 “Frontiers of RNAi”) and Prix Liliane Bettencourt for Life Sciences. ",

year = "2010",

month = jun,

doi = "10.1016/j.cell.2010.04.042",

language = "English (US)",

volume = "141",

pages = "956--969",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "6",

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TY - JOUR

T1 - LINE-1 activity in facultative heterochromatin formation during X chromosome inactivation

AU - Chow, Jennifer C.

AU - Ciaudo, Constance

AU - Fazzari, Melissa J.

AU - Mise, Nathan

AU - Servant, Nicolas

AU - Glass, Jacob L.

AU - Attreed, Matthew

AU - Avner, Philip

AU - Wutz, Anton

AU - Barillot, Emmanuel

AU - Greally, John M.

AU - Voinnet, Olivier

AU - Heard, Edith

N1 - Funding Information: We would like to thank members of the Heard laboratory and Vincent Colot for critical reading of the manuscript; John Goodier and Deborah Bourc'his for advice and mouse LINE-1 plasmid constructs; and Valerie Cognat (IBMP) for expert bioinformatics analyses. This work was supported by grants from the ANR (“RNA ES”; to both E.H. and O.V.); FRM (Equipe FRM to E.H. and FRM fellowship to J.C.); HEROIC, an Integrated Project funded by the European Union under the 6th Framework Programme (LSHG-CT-2005-018883); and Canceropole IdF (GET project) to E.H; O.V.'s laboratory was further supported by an ERC Young Investigator Award (Grant ERC-210890 “Frontiers of RNAi”) and Prix Liliane Bettencourt for Life Sciences.

PY - 2010/6

Y1 - 2010/6

N2 - During X chromosome inactivation (XCI), Xist RNA coats and silences one of the two X chromosomes in female cells. Little is known about how XCI spreads across the chromosome, although LINE-1 elements have been proposed to play a role. Here we show that LINEs participate in creating a silent nuclear compartment into which genes become recruited. A subset of young LINE-1 elements, however, is expressed during XCI, rather than being silenced. We demonstrate that such LINE expression requires the specific heterochromatic state induced by Xist. These LINEs often lie within escape-prone regions of the X chromosome, but close to genes that are subject to XCI, and are associated with putative endo-siRNAs. LINEs may thus facilitate XCI at different levels, with silent LINEs participating in assembly of a heterochromatic nuclear compartment induced by Xist, and active LINEs participating in local propagation of XCI into regions that would otherwise be prone to escape. PaperClip:

AB - During X chromosome inactivation (XCI), Xist RNA coats and silences one of the two X chromosomes in female cells. Little is known about how XCI spreads across the chromosome, although LINE-1 elements have been proposed to play a role. Here we show that LINEs participate in creating a silent nuclear compartment into which genes become recruited. A subset of young LINE-1 elements, however, is expressed during XCI, rather than being silenced. We demonstrate that such LINE expression requires the specific heterochromatic state induced by Xist. These LINEs often lie within escape-prone regions of the X chromosome, but close to genes that are subject to XCI, and are associated with putative endo-siRNAs. LINEs may thus facilitate XCI at different levels, with silent LINEs participating in assembly of a heterochromatic nuclear compartment induced by Xist, and active LINEs participating in local propagation of XCI into regions that would otherwise be prone to escape. PaperClip:

KW - DNA

KW - Devbio

KW - RNA

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U2 - 10.1016/j.cell.2010.04.042

DO - 10.1016/j.cell.2010.04.042

M3 - Article

C2 - 20550932

AN - SCOPUS:77953703353

SN - 0092-8674

VL - 141

SP - 956

EP - 969

JO - Cell

JF - Cell

IS - 6

ER -

LINE-1 activity in facultative heterochromatin formation during X chromosome inactivation

Abstract

Keywords

ASJC Scopus subject areas

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