Lectin-Resistant CHO Glycosylation Mutants

Santosh Kumar Patnaik, Pamela Stanley

Research output: Chapter in Book/Report/Conference proceedingChapter

168 Scopus citations


Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.

Original languageEnglish (US)
Title of host publicationGlycomics
EditorsMinoru Fukuda
Number of pages24
StatePublished - 2006
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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