TY - JOUR
T1 - Lectin-resistant CHO cells
T2 - Selection of new mutant phenotypes
AU - Stanley, Pamela
PY - 1983/9
Y1 - 1983/9
N2 - Cytotoxic plant lectins select for mutants which exhibit unique structural changes in surface carbohydrates reflecting specific defects in glycosylation reactions. However, lectins are not highly specific selective agents and, as a result, only the most frequently occurring mutants are obtained from single lectin selections. We have previously shown that the specificity of lectin selections may be improved by utilizing a combination of lectins added together or sequentially. This strategy has now been further exploited in the search for novel lectin-resistant mutants of Chinese hamster ovary cells. Five new LeeR phenotypes have been uncovered. One belongs to a new, recessive complementation group, two behave dominantly in somatic cell hybrids, and the remaining two appear to represent new phenotypes which fall into previously described complementation groups.
AB - Cytotoxic plant lectins select for mutants which exhibit unique structural changes in surface carbohydrates reflecting specific defects in glycosylation reactions. However, lectins are not highly specific selective agents and, as a result, only the most frequently occurring mutants are obtained from single lectin selections. We have previously shown that the specificity of lectin selections may be improved by utilizing a combination of lectins added together or sequentially. This strategy has now been further exploited in the search for novel lectin-resistant mutants of Chinese hamster ovary cells. Five new LeeR phenotypes have been uncovered. One belongs to a new, recessive complementation group, two behave dominantly in somatic cell hybrids, and the remaining two appear to represent new phenotypes which fall into previously described complementation groups.
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U2 - 10.1007/BF01574260
DO - 10.1007/BF01574260
M3 - Article
C2 - 6623313
AN - SCOPUS:0021029861
SN - 0098-0366
VL - 9
SP - 593
EP - 608
JO - Somatic Cell Genetics
JF - Somatic Cell Genetics
IS - 5
ER -