Joint composite-rotation adiabatic-sweep isotope filtration

Elizabeth R. Valentine, Fabien Ferrage, Francesca Massi, David Cowburn, Arthur G. Palmer

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Joint composite-rotation adiabatic-sweep isotope filters are derived by combining the composite-rotation [Stuart AC et al. (1999) J Am Chem Soc 121: 5346-5347] and adiabatic-sweep [Zwahlen C et al. (1997) J Am Chem Soc 119:6711-6721; Kup̂e E, Freeman R (1997) J Magn Reson 127:36-48] approaches. The joint isotope filters have improved broadband filtration performance, even for extreme values of the one-bond 1H-13C scalar coupling constants in proteins and RNA molecules. An average Hamiltonian analysis is used to describe evolution of the heteronuclear scalar coupling interaction during the adiabatic sweeps within the isotope filter sequences. The new isotope filter elements permit improved selective detection of NMR resonance signals originating from 1H spins attached to an unlabeled natural abundance component of a complex in which the other components are labeled with 13C and 15N isotopes.

Original languageEnglish (US)
Pages (from-to)11-22
Number of pages12
JournalJournal of Biomolecular NMR
Issue number1
StatePublished - May 2007


  • Composite pulse
  • J-filter
  • Structure determination
  • Vts SAM domain

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy


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