Isolation of Extracellular Vesicles from Candida auris

Daniel Zamith-Miranda; Lysangela R. Alves; Marcio L. Rodrigues; Leonardo Nimrichter; Joshua D. Nosanchuk

doi:10.1007/978-1-0716-2417-3_13

Isolation of Extracellular Vesicles from Candida auris

Daniel Zamith-Miranda, Lysangela R. Alves, Marcio L. Rodrigues, Leonardo Nimrichter, Joshua D. Nosanchuk

Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

Extracellular vesicles (EVs) are structures released by a variety of cells from all kingdoms of life. EVs are typically involved in communication between tissues and organs, between distinct organisms, or inside microbial communities. The plasticity of these structures is reflected in the range of biological effects they are able to induce or inhibit. The study of fungal EVs is relatively new with the first report in 2007, but investigators have already demonstrated in several model systems that fungal EVs significantly modulate the host immune system and that the immunogenic materials in EV can be harnessed as vaccination platforms. This chapter describes the two main procedures used to isolate EVs from an emerging pathogenic fungus, Candida auris.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	173-178
Number of pages	6
DOIs	https://doi.org/10.1007/978-1-0716-2417-3_13
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2517
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Candida auris
Culture
Extracellular vesicles
Fungal

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-0716-2417-3_13

Cite this

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title = "Isolation of Extracellular Vesicles from Candida auris",

abstract = "Extracellular vesicles (EVs) are structures released by a variety of cells from all kingdoms of life. EVs are typically involved in communication between tissues and organs, between distinct organisms, or inside microbial communities. The plasticity of these structures is reflected in the range of biological effects they are able to induce or inhibit. The study of fungal EVs is relatively new with the first report in 2007, but investigators have already demonstrated in several model systems that fungal EVs significantly modulate the host immune system and that the immunogenic materials in EV can be harnessed as vaccination platforms. This chapter describes the two main procedures used to isolate EVs from an emerging pathogenic fungus, Candida auris.",

keywords = "Candida auris, Culture, Extracellular vesicles, Fungal",

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doi = "10.1007/978-1-0716-2417-3_13",

language = "English (US)",

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AU - Alves, Lysangela R.

AU - Rodrigues, Marcio L.

AU - Nimrichter, Leonardo

AU - Nosanchuk, Joshua D.

PY - 2022

Y1 - 2022

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AB - Extracellular vesicles (EVs) are structures released by a variety of cells from all kingdoms of life. EVs are typically involved in communication between tissues and organs, between distinct organisms, or inside microbial communities. The plasticity of these structures is reflected in the range of biological effects they are able to induce or inhibit. The study of fungal EVs is relatively new with the first report in 2007, but investigators have already demonstrated in several model systems that fungal EVs significantly modulate the host immune system and that the immunogenic materials in EV can be harnessed as vaccination platforms. This chapter describes the two main procedures used to isolate EVs from an emerging pathogenic fungus, Candida auris.

KW - Candida auris

KW - Culture

KW - Extracellular vesicles

KW - Fungal

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PB - Humana Press Inc.

ER -

Isolation of Extracellular Vesicles from Candida auris

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

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