Isolation and analysis of primitive hemopoietic progenitor cells on the basis of differential expression of Qa-m7 antigen

I. Bertoncello, S. H. Bartelmez, T. R. Bradley, E. R. Stanley, R. A. Harris, M. S. Sandrin, A. B. Kriegler, I. K. McNiece, S. D. Hunter, G. S. Hodgson

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


In the presence of the hemopoietic growth factor CSF-1, the later committed cells of the macrophage lineage can be detected by their ability to form small colonies in clonal agar culture (CFC(CSF-1)). Synergistic factors have been described that in combination with CSF-1 stimulate developmentally early hemopoietic progenitor cells of high proliferative potential (HPP-CFC). By using a monoclonal antibody to the Qa-m7 antigenic determinant, we investigated and compared the expression of Qa-m7 on CFC(CSF-1) and on HPP-CFC of two types that grow in response to either 1) CSF-1 plus synergistic factor from human placenta-conditioned medium (HPP-CFC(Hplac+CSF-1)) or 2) CSF-1 plus synergistic factor from conditioned medium of the WEHI-3 myelomonocytic cell line (HPP-CFC(W+CSF-1)). We have shown that HPP-CFC of both types express relatively more Qa-m7 antigen then CFC(CSF-1) and can be separated and enriched on this basis by discontinuous buoyant density centrifugation and fluorescence-activated cell sorting of normal bone marrow. Significant enrichments of HPP-CFC(HPlac+CSF-1) (43.5-fold) and HPP-CFC(W+CSF-1) (28.8-fold) have been achieved with cloning efficiencies of HPP-CFC in the most enriched fractions reaching 4 to 5%. These results clearly illustrate the fact that there are populations of progenitor cells from normal, unperturbed bone marrow that strictly require a combination of two hemopoietic growth factors (CSF-1 plus synergistic factor) in order to be detected.

Original languageEnglish (US)
Pages (from-to)3219-3224
Number of pages6
JournalJournal of Immunology
Issue number9
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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