Intravital two-photon excitation microscopy in neuroscience: General concepts and applications

J. Tiago Gonçalves; Ricardo Mostany

doi:10.1007/978-94-017-9361-2_1

Intravital two-photon excitation microscopy in neuroscience: General concepts and applications

J. Tiago Gonçalves, Ricardo Mostany

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Multiphoton excitation microscopy has revolutionized biomedical research during the last two decades by enabling high resolution fluorescent microscopy in intact tissues. This feature makes two-photon excitation (2PE) microscopy ideal for intravital imaging of neural tissue, permitting the observation of structural and functional neuronal dynamics in undisrupted neural circuits. Here we review the fundamental concepts of intravital 2PE microscopy, describe methods and techniques associated with it, and highlight the most significant findings reported on neuron and glia structure dynamics as well as on neuronal activity using this in vivo imaging technique.

Original language	English (US)
Title of host publication	Advances in Intravital Microscopy
Subtitle of host publication	From Basic to Clinical Research
Publisher	Springer Netherlands
Pages	1-23
Number of pages	23
ISBN (Electronic)	9789401793612
ISBN (Print)	9789401793605
DOIs	https://doi.org/10.1007/978-94-017-9361-2_1
State	Published - Jan 1 2014
Externally published	Yes

Keywords

Axonal bouton
Calcium imaging
Chronic in vivo imaging
Cranial window
Deep tissue imaging
Dendritic spine
Fluorophore
GECI
Glia
Optogenetics
Pyramidal neuron
Thinned-skull

ASJC Scopus subject areas

Medicine(all)

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10.1007/978-94-017-9361-2_1

Cite this

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abstract = "Multiphoton excitation microscopy has revolutionized biomedical research during the last two decades by enabling high resolution fluorescent microscopy in intact tissues. This feature makes two-photon excitation (2PE) microscopy ideal for intravital imaging of neural tissue, permitting the observation of structural and functional neuronal dynamics in undisrupted neural circuits. Here we review the fundamental concepts of intravital 2PE microscopy, describe methods and techniques associated with it, and highlight the most significant findings reported on neuron and glia structure dynamics as well as on neuronal activity using this in vivo imaging technique.",

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AU - Mostany, Ricardo

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AB - Multiphoton excitation microscopy has revolutionized biomedical research during the last two decades by enabling high resolution fluorescent microscopy in intact tissues. This feature makes two-photon excitation (2PE) microscopy ideal for intravital imaging of neural tissue, permitting the observation of structural and functional neuronal dynamics in undisrupted neural circuits. Here we review the fundamental concepts of intravital 2PE microscopy, describe methods and techniques associated with it, and highlight the most significant findings reported on neuron and glia structure dynamics as well as on neuronal activity using this in vivo imaging technique.

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KW - Optogenetics

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Intravital two-photon excitation microscopy in neuroscience: General concepts and applications

Abstract

Keywords

ASJC Scopus subject areas

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