Intravital two-photon excitation microscopy in neuroscience: General concepts and applications

J. Tiago Gonçalves, Ricardo Mostany

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Multiphoton excitation microscopy has revolutionized biomedical research during the last two decades by enabling high resolution fluorescent microscopy in intact tissues. This feature makes two-photon excitation (2PE) microscopy ideal for intravital imaging of neural tissue, permitting the observation of structural and functional neuronal dynamics in undisrupted neural circuits. Here we review the fundamental concepts of intravital 2PE microscopy, describe methods and techniques associated with it, and highlight the most significant findings reported on neuron and glia structure dynamics as well as on neuronal activity using this in vivo imaging technique.

Original languageEnglish (US)
Title of host publicationAdvances in Intravital Microscopy
Subtitle of host publicationFrom Basic to Clinical Research
PublisherSpringer Netherlands
Number of pages23
ISBN (Electronic)9789401793612
ISBN (Print)9789401793605
StatePublished - Jan 1 2014
Externally publishedYes


  • Axonal bouton
  • Calcium imaging
  • Chronic in vivo imaging
  • Cranial window
  • Deep tissue imaging
  • Dendritic spine
  • Fluorophore
  • GECI
  • Glia
  • Optogenetics
  • Pyramidal neuron
  • Thinned-skull

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Intravital two-photon excitation microscopy in neuroscience: General concepts and applications'. Together they form a unique fingerprint.

Cite this