TY - JOUR
T1 - Interleukin-17A as a Biomarker for Bovine Tuberculosis
AU - Waters, W. Ray
AU - Maggioli, Mayara F.
AU - Palmer, Mitchell V.
AU - Thacker, Tyler C.
AU - McGill, Jodi L.
AU - Vordermeier, H. Martin
AU - Berney-Meyer, Linda
AU - Jacobs, William R.
AU - Larsen, Michelle H.
N1 - Publisher Copyright:
Copyright © 2016 American Society for Microbiology. All Rights Reserved.
PY - 2016/2
Y1 - 2016/2
N2 - T helper 17 (Th17)-associated cytokines are integral to the immune responses to tuberculosis, initiating both protective and harmful inflammatory responses. The aim of the present study was to evaluate applied aspects of interleukin-17 (IL-17) biology in the context of Mycobacterium bovis infection of cattle. Using transcriptome sequencing (RNA-Seq), numerous Th17-associated cytokine genes (including IL-17A, IL-17F, IL-22, IL-19, and IL-27) were upregulated>9-fold in response to purified protein derivative stimulation of peripheral blood mononuclear cells from experimentally M. bovis-infected cattle. Protective vaccines elicited IL-17A, IL-17F, IL-22, and IL-27 responses. Reduced IL-17A responses by vaccine recipients, compared to nonvaccinated animals, at 2.5 weeks after M. bovis challenge correlated with reduced disease burdens. Additionally, IL-17A and interferon gamma (IFN-γ) responses were highly correlated and exhibited similar diagnostic capacities. The present findings support the use of Th17-associated cytokines as biomarkers of infection and protection in the immune responses to bovine tuberculosis.
AB - T helper 17 (Th17)-associated cytokines are integral to the immune responses to tuberculosis, initiating both protective and harmful inflammatory responses. The aim of the present study was to evaluate applied aspects of interleukin-17 (IL-17) biology in the context of Mycobacterium bovis infection of cattle. Using transcriptome sequencing (RNA-Seq), numerous Th17-associated cytokine genes (including IL-17A, IL-17F, IL-22, IL-19, and IL-27) were upregulated>9-fold in response to purified protein derivative stimulation of peripheral blood mononuclear cells from experimentally M. bovis-infected cattle. Protective vaccines elicited IL-17A, IL-17F, IL-22, and IL-27 responses. Reduced IL-17A responses by vaccine recipients, compared to nonvaccinated animals, at 2.5 weeks after M. bovis challenge correlated with reduced disease burdens. Additionally, IL-17A and interferon gamma (IFN-γ) responses were highly correlated and exhibited similar diagnostic capacities. The present findings support the use of Th17-associated cytokines as biomarkers of infection and protection in the immune responses to bovine tuberculosis.
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U2 - 10.1128/CVI.00637-15
DO - 10.1128/CVI.00637-15
M3 - Article
C2 - 26677202
AN - SCOPUS:84958642043
SN - 1556-6811
VL - 23
SP - 168
EP - 180
JO - Clinical and Vaccine Immunology
JF - Clinical and Vaccine Immunology
IS - 2
ER -
