Interaction of the glycocalyx with the actin cytoskeleton

Mia M. Thi; Eno E. Ebong; David C. Spray; Sylvia O. Suadicani

doi:10.1007/978-1-62703-266-7_3

Interaction of the glycocalyx with the actin cytoskeleton

Mia M. Thi, Eno E. Ebong, David C. Spray, Sylvia O. Suadicani

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

In this chapter, we summarize and describe various methods that have been used to detect glycocalyx and to study the interactions between glycocalyx and actin cytoskeleton with the emphasis in the nervous tissue. We highlight two methodologies to analyze the direct interaction between glycocalyx and actin cytoskeleton: one method focusing on visualization using immunostaining and confocal microscopy techniques and the other focusing on direct protein-protein interactions using co-immunoprecipitation techniques.

Original language	English (US)
Title of host publication	The Cytoskeleton
Subtitle of host publication	Imaging, Isolation, and Interaction
Publisher	Humana Press Inc.
Pages	43-62
Number of pages	20
ISBN (Print)	9781627032650
DOIs	https://doi.org/10.1007/978-1-62703-266-7_3
State	Published - 2013

Publication series

Name	Neuromethods
Volume	79
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Keywords

Actin cytoskeleton
Astrocyte
Chondroitin sulfate
Heparan sulfate
Hyaluronic acid

ASJC Scopus subject areas

Psychiatry and Mental health
General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology
General Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-62703-266-7_3

Cite this

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title = "Interaction of the glycocalyx with the actin cytoskeleton",

abstract = "In this chapter, we summarize and describe various methods that have been used to detect glycocalyx and to study the interactions between glycocalyx and actin cytoskeleton with the emphasis in the nervous tissue. We highlight two methodologies to analyze the direct interaction between glycocalyx and actin cytoskeleton: one method focusing on visualization using immunostaining and confocal microscopy techniques and the other focusing on direct protein-protein interactions using co-immunoprecipitation techniques.",

keywords = "Actin cytoskeleton, Astrocyte, Chondroitin sulfate, Heparan sulfate, Hyaluronic acid",

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T1 - Interaction of the glycocalyx with the actin cytoskeleton

AU - Thi, Mia M.

AU - Ebong, Eno E.

AU - Spray, David C.

AU - Suadicani, Sylvia O.

PY - 2013

Y1 - 2013

N2 - In this chapter, we summarize and describe various methods that have been used to detect glycocalyx and to study the interactions between glycocalyx and actin cytoskeleton with the emphasis in the nervous tissue. We highlight two methodologies to analyze the direct interaction between glycocalyx and actin cytoskeleton: one method focusing on visualization using immunostaining and confocal microscopy techniques and the other focusing on direct protein-protein interactions using co-immunoprecipitation techniques.

AB - In this chapter, we summarize and describe various methods that have been used to detect glycocalyx and to study the interactions between glycocalyx and actin cytoskeleton with the emphasis in the nervous tissue. We highlight two methodologies to analyze the direct interaction between glycocalyx and actin cytoskeleton: one method focusing on visualization using immunostaining and confocal microscopy techniques and the other focusing on direct protein-protein interactions using co-immunoprecipitation techniques.

KW - Actin cytoskeleton

KW - Astrocyte

KW - Chondroitin sulfate

KW - Heparan sulfate

KW - Hyaluronic acid

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DO - 10.1007/978-1-62703-266-7_3

M3 - Chapter

AN - SCOPUS:84875468317

SN - 9781627032650

T3 - Neuromethods

SP - 43

EP - 62

BT - The Cytoskeleton

PB - Humana Press Inc.

ER -

Interaction of the glycocalyx with the actin cytoskeleton

Abstract

Publication series

Keywords

ASJC Scopus subject areas

UN SDGs

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