Insulin/NFκB protects against ischemia-induced necrotic cardiomyocyte death

Ariel Díaz, Claudio Humeres, Verónica González, María Teresa Gómez, Natalia Montt, Gina Sanchez, Mario Chiong, Lorena García

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


In the heart, insulin controls key functions such as metabolism, muscle contraction and cell death. However, all studies have been focused on insulin action during reperfusion. Here we explore the cardioprotective action of this hormone during ischemia. Rat hearts were perfused ex vivo with an ischemia/reperfusion Langendorff model in absence or presence of insulin. Additionally, cultured rat cardiomyocytes were exposed to simulated ischemia in the absence or presence of insulin. Cytoprotective effects were measured by myocardial infarct size, trypan blue exclusion, released LDH and DNA fragmentation by flow cytometry. We found that insulin protected against cardiac ischemia ex vivo and in vitro. Moreover, insulin protected cardiomyocytes from simulated ischemia by reducing necrotic cell death. Protective effects of insulin were dependent of Akt and NFκB. These novel results show that insulin reduces ischemia-induced cardiomyocyte necrosis through an Akt/NF-κB dependent mechanism. These novel findings clarify the role of insulin during ischemia and further support its use in early GIK perfusion to treat myocardial infarction.

Original languageEnglish (US)
Pages (from-to)451-457
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Nov 13 2015
Externally publishedYes


  • Cell death
  • Cell signaling
  • Heart
  • Insulin
  • Ischemia

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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