Insulin enhances glucose-stimulated insulin secretion in healthy humans

Clara Bouche, Ximena Lopez, Amy Fleischman, Aaron M. Cypess, Sheila O'Shea, Darko Stefanovski, Richard N. Bergman, Eduard Rogatsky, Daniel T. Stein, C. Ronald Kahn, Rohit N. Kulkarni, Allison B. Goldfine

Research output: Contribution to journalArticlepeer-review

71 Scopus citations


Islet β-cells express both insulin receptors and insulin-signaling proteins. Recent evidence from rodents in vivo and from islets isolated from rodents or humans suggests that the insulin signaling pathway is physiologically important for glucose sensing. We evaluated whether insulin regulates β-cell function in healthy humans in vivo. Glucose-induced insulin secretion was assessed in healthy humans following 4-h saline (low insulin/sham clamp) or isoglycemic-hyperinsulinemic (high insulin) clamps using B28-Asp insulin that could be immunologically distinguished from endogenous insulin. Insulin and C-peptide clearance were evaluated to understand the impact of hyperinsulinemia on estimates of β-cell function. Preexposure to exogenous insulin increased the endogenous insulin secretory response to glucose by ≈40%. C-peptide response also increased, although not to the level predicted by insulin. Insulin clearance was not saturated at hyperinsulinemia, but metabolic clearance of C-peptide, assessed by infusion of stable isotope-labeled C-peptide, increased modestly during hyperinsulinemic clamp. These studies demonstrate that insulin potentiates glucose-stimulated insulin secretion in vivo in healthy humans. In addition, hyperinsulinemia increases C-peptide clearance, which may lead to modest underestimation of β-cell secretory response when using these methods during prolonged dynamic testing.

Original languageEnglish (US)
Pages (from-to)4770-4775
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10
StatePublished - Mar 9 2010
Externally publishedYes


  • Beta cell
  • C-peptide
  • Insulin clearance
  • Insulin resistance
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Insulin enhances glucose-stimulated insulin secretion in healthy humans'. Together they form a unique fingerprint.

Cite this