Abstract
Rat adipose cells treated with Staphylococcus aureus α-toxin are permeable and retain their ability to respond to insulin after hormone treatment. The GLUT 4 glucose transporter isoform, specific to fat and muscle cells, is translocated normally from low density microsomes to the plasma membrane in permeabilized cells. Addition of guanosine 5′-O-(3-thiotriphosphate), guanylyl imidodiphosphate, or guanylyl β,γ-methylenediphosphate to permeabilized adipocytes induces an insulin-like translocation of GLUT 4 to the plasma membrane; GTP or adenosine 5′-(β,γ-imino)triphosphate has no effect. No translocation of GLUT 4 is observed when GTP analogs are added to intact adipocytes. These results suggest the involvement of a GTP-binding protein in insulin-triggered recruitment of GLUT 4 to the cell surface.
Original language | English (US) |
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Pages (from-to) | 4037-4040 |
Number of pages | 4 |
Journal | Journal of Biological Chemistry |
Volume | 266 |
Issue number | 7 |
State | Published - Mar 5 1991 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology