Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation

B. F. Mackler; L. C. Altman; D. L. Rosenstreich; J. J. Oppenheim

doi:10.1038/249834a0

Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation

B. F. Mackler, L. C. Altman, D. L. Rosenstreich, J. J. Oppenheim

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Abstract

Two groups of human lymphocytes are distinguished on the basis of immune effector functions, membrane markers and in vitro mitogen reactivity. Thymus-derived (T) lymphocytes mediate cellular immunity either directly or by secreting pharmacologically active lymphokines¹, and are implicated in facilitating antibody responses². In contrast, bone marrow-derived (B) lymphocytes are thought to produce only antibodies. Studies in the guinea pig, however, indicate that, appropriately stimulated, B cells can produce macrophage migration inhibitory factor (MIF)³. We have now found that the erythrocyte-antibody (19S)-complement (EAC) rosetting method, used to obtain pure human peripheral blood B cells, activates these B cells to produce mononuclear cell chemotactic (CTX) and mitogenic (MF) lymphokines. Furthermore, we have investigated the mechanism of B cell activation by EAC and the effects of mitogenic lymphokines on human T and B lymphocytes.

Original language	English (US)
Pages (from-to)	834-837
Number of pages	4
Journal	Nature
Volume	249
Issue number	5460
DOIs	https://doi.org/10.1038/249834a0
State	Published - 1974
Externally published	Yes

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title = "Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation",

abstract = "Two groups of human lymphocytes are distinguished on the basis of immune effector functions, membrane markers and in vitro mitogen reactivity. Thymus-derived (T) lymphocytes mediate cellular immunity either directly or by secreting pharmacologically active lymphokines1, and are implicated in facilitating antibody responses2. In contrast, bone marrow-derived (B) lymphocytes are thought to produce only antibodies. Studies in the guinea pig, however, indicate that, appropriately stimulated, B cells can produce macrophage migration inhibitory factor (MIF)3. We have now found that the erythrocyte-antibody (19S)-complement (EAC) rosetting method, used to obtain pure human peripheral blood B cells, activates these B cells to produce mononuclear cell chemotactic (CTX) and mitogenic (MF) lymphokines. Furthermore, we have investigated the mechanism of B cell activation by EAC and the effects of mitogenic lymphokines on human T and B lymphocytes.",

author = "Mackler, {B. F.} and Altman, {L. C.} and Rosenstreich, {D. L.} and Oppenheim, {J. J.}",

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T1 - Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation

AU - Mackler, B. F.

AU - Altman, L. C.

AU - Rosenstreich, D. L.

AU - Oppenheim, J. J.

PY - 1974

Y1 - 1974

N2 - Two groups of human lymphocytes are distinguished on the basis of immune effector functions, membrane markers and in vitro mitogen reactivity. Thymus-derived (T) lymphocytes mediate cellular immunity either directly or by secreting pharmacologically active lymphokines1, and are implicated in facilitating antibody responses2. In contrast, bone marrow-derived (B) lymphocytes are thought to produce only antibodies. Studies in the guinea pig, however, indicate that, appropriately stimulated, B cells can produce macrophage migration inhibitory factor (MIF)3. We have now found that the erythrocyte-antibody (19S)-complement (EAC) rosetting method, used to obtain pure human peripheral blood B cells, activates these B cells to produce mononuclear cell chemotactic (CTX) and mitogenic (MF) lymphokines. Furthermore, we have investigated the mechanism of B cell activation by EAC and the effects of mitogenic lymphokines on human T and B lymphocytes.

AB - Two groups of human lymphocytes are distinguished on the basis of immune effector functions, membrane markers and in vitro mitogen reactivity. Thymus-derived (T) lymphocytes mediate cellular immunity either directly or by secreting pharmacologically active lymphokines1, and are implicated in facilitating antibody responses2. In contrast, bone marrow-derived (B) lymphocytes are thought to produce only antibodies. Studies in the guinea pig, however, indicate that, appropriately stimulated, B cells can produce macrophage migration inhibitory factor (MIF)3. We have now found that the erythrocyte-antibody (19S)-complement (EAC) rosetting method, used to obtain pure human peripheral blood B cells, activates these B cells to produce mononuclear cell chemotactic (CTX) and mitogenic (MF) lymphokines. Furthermore, we have investigated the mechanism of B cell activation by EAC and the effects of mitogenic lymphokines on human T and B lymphocytes.

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Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation

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