TY - JOUR
T1 - Inducible protein stabilization system in Leishmania mexicana
AU - Podešvová, Lucie
AU - Huang, Huan
AU - Yurchenko, Vyacheslav
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD.
AB - Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD.
KW - ecDHFR
KW - Leishmania mexicana
KW - Protein stabilization
KW - Trimethoprim
UR - http://www.scopus.com/inward/record.url?scp=85017158365&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017158365&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2017.03.008
DO - 10.1016/j.molbiopara.2017.03.008
M3 - Article
C2 - 28373094
AN - SCOPUS:85017158365
SN - 0166-6851
VL - 214
SP - 62
EP - 64
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
ER -