Increased plasma levels of NGAL, a marker of neutrophil activation, in patients with abdominal aortic aneurysm

Priscila Ramos-Mozo, Julio Madrigal-Matute, Melina Vega de Ceniga, Luis Miguel Blanco-Colio, Olivier Meilhac, Laurent Feldman, Jean Baptiste Michel, Paula Clancy, Jonathan Golledge, Paul E. Norman, Jesus Egido, José Luis Martin-Ventura

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


Objective: Neutrophil gelatinase-associated lipocalin (NGAL) plasma concentrations have been associated with cardiovascular diseases. We aimed to assess the association of NGAL with abdominal aortic aneurysm (AAA). Methods: NGAL concentrations were analyzed by Western blotting in conditioned medium of polymorphonuclear neutrophils (PMNs) from AAA patients (n=22) and controls (n=11), and also in aortic biopsies from AAA patients and healthy controls (n=10). Plasma NGAL concentrations were measured by ELISA in three groups of subjects from France (n=60), Spain (n=75) and Australia (n=100) and associated with AAA presence and growth. Results: PMNs isolated from AAA patients secreted significantly greater amounts of NGAL than PMNs from controls. Luminal thrombus released large amounts of NGAL compared to abluminal AAA thrombus, AAA wall and healthy aortic media. Plasma NGAL concentrations were significantly higher in patients with AAA than controls from France [115 (78-200) vs. 94 (72-114). ng/ml, p<0.001]. NGAL plasma concentrations in AAA patients from Spain correlated with other markers of thrombus activity (plasmin-antiplasmin complexes and D-dimer). Furthermore, a positive correlation between plasma NGAL and retrospective AAA growth (rho = 0.4, p=0.01) was observed, which remained significant after adjusting for other risk factors. Plasma NGAL was only weakly associated with prospective growth in both Spanish and Australian patients. Conclusions: NGAL is released by PMNs and by the luminal part of AAA thrombus. NGAL plasma levels were increased in AAA patients compared with healthy subjects and correlated with retrospective AAA growth. Further studies in larger subjects groups are needed to confirm the association between NGAL and AAA presence and growth.

Original languageEnglish (US)
Pages (from-to)552-556
Number of pages5
Issue number2
StatePublished - Feb 2012
Externally publishedYes


  • Abdominal aortic aneurysm
  • Biomarkers
  • Intraluminal thrombus
  • Neutrophil gelatinase-associated lipocalin
  • Polymorphonuclear neutrophils

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine


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