Abstract
HeLa cells, incubated with camptothecin during the G 1 phase of the cell cycle, show nuclear fluorescence with fluorescein-labeled antinucleoside antibodies. If the G 1 cells are washed free of the drug, the cells no longer demonstrate nuclear fluorescence. Since these antibodies react only with single-stranded DNA, the positive staining in camptothecin-treated G 1 cells suggests that the drug induces denatured regions in DNA. Fluorescent antinucleoside antibodies may be a useful technique for the observation of drug-induced changes in DNA during the G1 phase of the cell cycle.
Original language | English (US) |
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Pages (from-to) | 174-178 |
Number of pages | 5 |
Journal | Experimental Cell Research |
Volume | 86 |
Issue number | 1 |
DOIs | |
State | Published - May 1974 |
ASJC Scopus subject areas
- Cell Biology