TY - JOUR
T1 - Immunological evaluation of fusion protein of Brugia malayi abundant larval protein transcript-2 (BmALT-2) and Tuftsin in experimental mice model
AU - Paul, Rajkumar
AU - Ilamaran, Meganathan
AU - Khatri, Vishal
AU - Amdare, Nitin
AU - Reddy, Maryada Venkata Rami
AU - Kaliraj, Perumal
N1 - Funding Information:
This project was initially supported by Anna Centenary Fellowship from Anna University, Chennai (Lr. No. CR/ACRF/Jan.2012/30) followed by CSIR-JRF (NET) Fellowship (CSIR File No. 09/468(0467)/2012-EMR-I) from Council for Scientific and Industrial Research (CSIR), India. This is also partially funded by Indian Council of Medical Research (ICMR), India as ICMR Emeritus Medical Scientist Award to Dr. P. Kaliraj, Centre for Biotechnology, Anna University, Chennai (India). There is no role of funders in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Funding Information:
This project was initially supported by Anna Centenary Fellowship from Anna University, Chennai (Lr. No. CR/ACRF/Jan.2012/30 ) followed by CSIR-JRF (NET) Fellowship (CSIR File No. 09/468(0467)/2012-EMR-I ) from Council for Scientific and Industrial Research ( CSIR ), India. This is also partially funded by Indian Council of Medical Research ( ICMR ), India as ICMR Emeritus Medical Scientist Award to Dr. P. Kaliraj, Centre for Biotechnology, Anna University, Chennai (India). There is no role of funders in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2019
PY - 2019/2
Y1 - 2019/2
N2 - Introduction: Filariasis, a neglected tropical helminth disease needs vaccine besides mass drug administration for its successful eradication. Methods: An attempt was made to produce a fusion protein (P-TUFT-ALT-2) of abundant larval transcript protein-2 and Tuftsin to enhance its immunogenicity. The fusion construct was expressed in Pichia pastoris, a nonexpensive commercial expression system. This study focused on the evaluation of immunological response produced by P-TUFT-ALT-2 in Balb/c mice. Result and discussion: P-TUFT-ALT-2 showed an enhanced IgG peak titre compared to E. coli expressed E-ALT-2 and P. pastoris expressed P-ALT-2. IgG2b, IgG2a and IgG1 production were predominant indicating a balanced Th1/Th2 response. P-TUFT-ALT-2 also induced about 28% and 9.5% higher splenocyte proliferation over control and E-ALT-2 respectively. Splenocytes produced predominant IFN-γ followed by IL-5, IL-2 and IL-10 specifying a balanced Th1/Th2 response. P-TUFT-ALT-2 showed 55% to 80% with an average of 65% cytotoxicity in B. malayi L3 larvae in in vitro ADCC assay. Conclusion: This experiment validates P-TUFT-ALT-2 as a potential vaccine candidate for human lymphatic filariasis.
AB - Introduction: Filariasis, a neglected tropical helminth disease needs vaccine besides mass drug administration for its successful eradication. Methods: An attempt was made to produce a fusion protein (P-TUFT-ALT-2) of abundant larval transcript protein-2 and Tuftsin to enhance its immunogenicity. The fusion construct was expressed in Pichia pastoris, a nonexpensive commercial expression system. This study focused on the evaluation of immunological response produced by P-TUFT-ALT-2 in Balb/c mice. Result and discussion: P-TUFT-ALT-2 showed an enhanced IgG peak titre compared to E. coli expressed E-ALT-2 and P. pastoris expressed P-ALT-2. IgG2b, IgG2a and IgG1 production were predominant indicating a balanced Th1/Th2 response. P-TUFT-ALT-2 also induced about 28% and 9.5% higher splenocyte proliferation over control and E-ALT-2 respectively. Splenocytes produced predominant IFN-γ followed by IL-5, IL-2 and IL-10 specifying a balanced Th1/Th2 response. P-TUFT-ALT-2 showed 55% to 80% with an average of 65% cytotoxicity in B. malayi L3 larvae in in vitro ADCC assay. Conclusion: This experiment validates P-TUFT-ALT-2 as a potential vaccine candidate for human lymphatic filariasis.
KW - Abundant larval transcript-2
KW - Balb/c mice
KW - Lymphatic filariasis
KW - Pichia pastoris
KW - Tuftsin
KW - Vaccine candidate
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U2 - 10.1016/j.parepi.2019.e00092
DO - 10.1016/j.parepi.2019.e00092
M3 - Article
AN - SCOPUS:85061452483
SN - 2405-6731
VL - 4
JO - Parasite Epidemiology and Control
JF - Parasite Epidemiology and Control
M1 - e00092
ER -
