Immunological characterization of the endoproteases PC1 and PC2 in adrenal chromaffin granules and in the pituitary gland

Rudolf Kirchmair; Patricia Gee; Ruth Hogue-Angeletti; Andrea Laslop; Reiner Fischer-Colbrie; Hans Winkler

doi:10.1016/0014-5793(92)80560-4

Immunological characterization of the endoproteases PC1 and PC2 in adrenal chromaffin granules and in the pituitary gland

Rudolf Kirchmair, Patricia Gee, Ruth Hogue-Angeletti, Andrea Laslop, Reiner Fischer-Colbrie, Hans Winkler

Developmental & Molecular Biology

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Specific antisera against synthetic Fragments of the endoproteases, PC1 and PC2, were used to characterize these proteins. In one-dimensional immunoblots these antisera labelled components of 85 kDa for PC1 and of 70 kDa for PC2 in purified bovine chromaffin granules and anterior and posterior pituitary of ox and rat. In membranes of bovine chromaffin granules glycoprotein H was identified as the major PC2 immunoreactive spot. A major part of these endoproteases appeared membrane bound.

Original language	English (US)
Pages (from-to)	302-305
Number of pages	4
Journal	FEBS Letters
Volume	297
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(92)80560-4
State	Published - Feb 10 1992

Keywords

Adrenal medulla
Chromaffin granule
Endoprotease
PC1
PC2
Pituitary

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(92)80560-4

Cite this

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title = "Immunological characterization of the endoproteases PC1 and PC2 in adrenal chromaffin granules and in the pituitary gland",

abstract = "Specific antisera against synthetic Fragments of the endoproteases, PC1 and PC2, were used to characterize these proteins. In one-dimensional immunoblots these antisera labelled components of 85 kDa for PC1 and of 70 kDa for PC2 in purified bovine chromaffin granules and anterior and posterior pituitary of ox and rat. In membranes of bovine chromaffin granules glycoprotein H was identified as the major PC2 immunoreactive spot. A major part of these endoproteases appeared membrane bound.",

keywords = "Adrenal medulla, Chromaffin granule, Endoprotease, PC1, PC2, Pituitary",

author = "Rudolf Kirchmair and Patricia Gee and Ruth Hogue-Angeletti and Andrea Laslop and Reiner Fischer-Colbrie and Hans Winkler",

note = "Funding Information: Ackno~vledgements: This study was supported by the Fonds zur Ffrdcrun$ der wissenschaftlichen Forschung (Austria), by the Dr. Legerlotz.Stiftung and by fonds from the NIH (Grant NF 22697. R,H,A.),",

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T1 - Immunological characterization of the endoproteases PC1 and PC2 in adrenal chromaffin granules and in the pituitary gland

AU - Kirchmair, Rudolf

AU - Gee, Patricia

AU - Hogue-Angeletti, Ruth

AU - Laslop, Andrea

AU - Fischer-Colbrie, Reiner

AU - Winkler, Hans

N1 - Funding Information: Ackno~vledgements: This study was supported by the Fonds zur Ffrdcrun$ der wissenschaftlichen Forschung (Austria), by the Dr. Legerlotz.Stiftung and by fonds from the NIH (Grant NF 22697. R,H,A.),

PY - 1992/2/10

Y1 - 1992/2/10

N2 - Specific antisera against synthetic Fragments of the endoproteases, PC1 and PC2, were used to characterize these proteins. In one-dimensional immunoblots these antisera labelled components of 85 kDa for PC1 and of 70 kDa for PC2 in purified bovine chromaffin granules and anterior and posterior pituitary of ox and rat. In membranes of bovine chromaffin granules glycoprotein H was identified as the major PC2 immunoreactive spot. A major part of these endoproteases appeared membrane bound.

AB - Specific antisera against synthetic Fragments of the endoproteases, PC1 and PC2, were used to characterize these proteins. In one-dimensional immunoblots these antisera labelled components of 85 kDa for PC1 and of 70 kDa for PC2 in purified bovine chromaffin granules and anterior and posterior pituitary of ox and rat. In membranes of bovine chromaffin granules glycoprotein H was identified as the major PC2 immunoreactive spot. A major part of these endoproteases appeared membrane bound.

KW - Adrenal medulla

KW - Chromaffin granule

KW - Endoprotease

KW - PC1

KW - PC2

KW - Pituitary

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Immunological characterization of the endoproteases PC1 and PC2 in adrenal chromaffin granules and in the pituitary gland

Abstract

Keywords

ASJC Scopus subject areas

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